酵母合成和除草剂活性评估阿斯酸的酵母合成和除草剂活性评估
Zhenzhen Zhou1,2, Yuting Zhang1,2, Qi Wu1
1Provincial Key Laboratory of Agrobiology and Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu P. R. China.
Journal of agricultural and food chemistry
|November 8, 2024
概括
一种新的天然产品除草剂,阿斯酸 (AA),在酵母中成功合成. 这种可持续的方法使AAAA成为可能.
科学领域:
- 生物技术是生物技术.
- 自然产品化学 自然产品化学
- 农业科学 农业科学
背景情况:
- 酸 (AA) 是一种新型天然产品除草剂.
- 在植物中,AA的目标是二氧酸脱水酶.
- 开发AA的可持续生产方法对于AA的应用至关重要.
研究的目的:
- 建立一种可持续的生物合成途径,用于酵母中的阿斯酸 (AA).
- 识别和结合关键酶以获得最佳的AA生产.
- 评估AA在各种杂草物种上的除草剂效果.
主要方法:
- 在*Saccharomyces cerevisiae*中引入AA生物合成基因集群.
- 从各种真菌来源选基烯环酶.
- 优化发酵条件,以提高AA的生产.
- 对AA对杂草发芽和生长的抑制作用的评估.
主要成果:
- 使用来自*Aspergillus taichungensis*的*sesquiterpene cyclase和来自*Penicillium brasilianum*的*P450s*实现了最佳的AA合成.
- 在优化酵母发酵中获得的最高AA标位为33.21 mg/L.
- 艾亚证明了强烈的抑制发芽的三色,波尔图拉卡奥莱拉西亚,Bidenspilosa,Lolium perenne和Leptochloa chinensis.
- AA抑制了芽和根的生长,对根的影响更为明显.
结论:
- 成功开发了一种用于酵母中AA生物合成的可持续方法.
- 工程酵母系统为AA生产提供了一个可行的平台.
- 亚斯酸显示出作为一种用于杂草控制的预出现除草剂的显著潜力.
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