来自软骨祖先的细胞外囊泡刺激II型原体的表达和伤口愈合在阴茎细胞
Daniel J Betensky1, Maxwell D Chen1, Jay Trivedi1
1Department of Orthopaedics, Brown University and Rhode Island Hospital, Providence, Rhode Island, USA.
概括
来自软骨的原始细胞外细胞囊泡 (CPCs-EVs) 通过刺激体基质基质生产和改善细胞修复来增强阴囊愈合. 在促进阴茎细胞功能方面,CPC-EV显著优于其他细胞衍生的EV.
科学领域:
- 整形医生 整形医生 整形医生
- 再生医学是一种再生医学.
- 细胞生物学 细胞生物学
背景情况:
- 膝关节半关节撕裂往往治愈不好,风险是创伤后关节炎.
- 人类软骨衍生原生细胞 (CPC) 促进阴囊愈合,但机制尚不清楚.
- 细胞外囊泡 (EVs) 是细胞间通信的关键媒介.
研究的目的:
- 为了研究CPC衍生的EVs对本地阴茎细胞的副效应.
- 为了确定CPC-EV是否可以刺激阴茎愈合和阴茎发育.
- 将CPC-EV与来自其他细胞来源的EV的疗效进行比较.
主要方法:
- 通过超离心,将EVs与人体CPC和骨髓衍生的树皮细胞分离.
- 描述EV大小,蛋白质表达 (西方斑) 和量化.
- 测定阴茎纤维肌细胞代谢活性 (MTT,ATP),二维伤口愈合和基因表达 (RT-qPCR).
主要成果:
- CPCs生产了表达CD9,CD81和HSP70.0的电动汽车.
- 与对照人群相比,CPC-EV治疗显著改善了阴茎纤维粒细胞的二维伤口愈合.
- CPC-EVs增加了II型原蛋白mRNA表达,表明增强了体生成.
结论:
- 由CPC衍生的EVs刺激阴茎细胞中体基质生产和伤口愈合.
- 确定了一个最佳剂量为1.0×107颗粒子/毫升的CPC-EV.
- 与MSC-EV相比,CPC-EV在阴茎修复方面显示出更好的治疗潜力.
相关概念视频
Phases of Wound Repair
5.9K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
5.9K
Fibril-associated Collagen
2.5K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
2.5K
Growth of Cartilage and Bone Tissue
3.1K
Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...
3.1K
Clinical Applications of Epidermal Stem Cells
2.7K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.7K
Extracellular Matrix
2.8K
Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
2.8K


