相关实验视频

Updated: Jun 8, 2025

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Published on: August 25, 2018

MsERF17 在干旱条件下促进乙烯诱导的安托西亚尼生物合成在马卢斯壮观的叶子叶子

Yaping Xu¹, Yixin Liu¹, Lu Yue¹

¹College of Landscape Architecture and Art, Northwest A&F University, Yangling, China.

Plant, cell & environment

|November 8, 2024

概括

干旱压力触发了Malus spectabilis叶子中的素积累,由乙烯反应因子MsERF17驱动. 这一因素激活了关键基因,揭示了植物中一种新的干旱反应途径.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

干旱显著影响植物生理学,包括氨酸生物合成,但分子机制尚未完全理解.
乙烯反应因子 (ERF) 是已知的植物对环境压力和二次代谢物产生反应的调节者.

研究的目的:

调查乙烯反应因子 (ERF) 在干旱诱导的Malus spectabilis中安素生物合成中的作用.
阐明 MsERF17 调节干旱压力下的安托西亚宁积累的分子机制.

主要方法:

在干旱条件下分析叶子颜色变化和酸含量.
对MsERF17.进行基因表达分析 (RT-qPCR) 和促进体结合试验 (EMSA).
在M. spectabilis组织中过渡性基因表达 (过度表达和VIGS).
在M. spectabilis calli.的稳定转化.

主要成果:

干旱导致M. spectabilis的叶子颜色从绿色转变为红色,这是由于氨-3,5-二甲化和氨-3-O-氨化的增加.
鉴定出MsERF17是干旱诱导的氨酸生物合成的关键积极调节者.
MsERF17直接与MsbHLH3和MsF3'H的促进体结合,激活它们的表达.
功能性研究证实了MsERF17在促进安托氨酸合成中的作用.

结论:

MsERF17在干旱应激反应中发挥着至关重要的作用,通过促进M. spectabilis中安东素积累,促进干旱应激反应.
乙烯诱导的 MsERF17 通过激活 MsbHLH3 和 MsF3'H 调节了氨酸生物合成.
这项研究为植物适应干旱压力的分子基础提供了新的见解.

关键词:

马卢斯·斯帕塔比利斯 (马卢斯·斯帕塔比利斯) 是一个壮观的动物.MsERF17 在线观看安托西亚宁合成的合成干旱干旱干旱干旱乙烯乙烯的使用方法

更多相关视频

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

Optical Clearing of Plant Tissues for Fluorescence Imaging

Optical Clearing of Plant Tissues for Fluorescence Imaging

Published on: January 5, 2022

相关概念视频

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

Regulation of Transpiration by Stomata

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.

Responses to Drought and Flooding

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.

Responses to Salt Stress

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...