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在多发性硬化症患者中 lncRNA A2M-AS1基因表达的初步评估

Shaghayegh Mohammadi¹, Tahereh Sadeghiyan¹, Mohammad Rezaei²

¹Department of Genetics, Faculty of Biology Sciences and Technology, Shahid Ashrafi Esfahani, Isfahan, Iran.

Advanced biomedical research

|November 8, 2024

概括

长非编码RNA (lncRNA) A2M-AS1的减少表达与多发性硬化症 (MS) 有关. 这种 lncRNA A2M-AS1 显示了作为诊断生物标志物在患者中识别多发性硬化症的潜力.

科学领域:

神经科学是一个神经科学.
遗传学遗传学是一个
生物标志物发现发现

背景情况:

多发性硬化症 (MS) 是全球主要的神经退行性疾病之一.
基因表达分析对于理解和预防MS等疾病至关重要.
非编码RNA因其在疾病发病过程中的作用以及作为潜在的诊断工具而越来越受认可.

研究的目的:

在MS患者中研究长非编码RNA (lncRNA) A2M-AS1的表达.
评估lncRNA A2M-AS1作为MS诊断生物标志物的潜力.
探索涉及MS中非编码RNA的基因相互作用.

主要方法:

利用来自GEO数据库的微阵列数据来分析MS患者的A2M-AS1表达.
使用 lncRResearch 数据库进行了 lncRNA-mRNA 相互作用分析.
在20个MS和20个健康样本中通过定量逆转录酶聚合酶链反应 (RT-qPCR) 验证的A2M-AS1表达变化.

主要成果:

与健康对照组相比,在MS患者样本中发现A2M-AS1基因表达显著下降.
接收器操作特征 (ROC) 分析表明,lncRNA A2M-AS1可以作为可靠的诊断生物标志物来区分多发性硬化症患者.
生物信息学和实验室分析证实了这些发现.

结论:

减少lncRNA A2M-AS1的表达与患MS的风险增加有关.
lncRNA A2M-AS1是多发性硬化症的一个有前途的诊断生物标志物.
对非编码RNA的进一步研究可能为MS诊断和治疗提供新的途径.

关键词:

生物标志物生物标志物基因表达分析基因表达分析长非编码RNA是什么意思多发性硬化症多发性硬化症

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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...