相关实验视频

Updated: Jun 28, 2026

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

在同轴3D生物打印结构中使用超声响应生物进行远程控制的基因传递

Mary K Lowrey^1,2, Holly Day^1,2, Kevin J Schilling^1,2

¹Biomedical Engineering Department, Oregon Health and Science University, Portland, OR 97201 USA.

Cellular and molecular bioengineering

|November 8, 2024

概括

这项研究引入了一种新的3D生物打印方法,使用超声响应基因传递生物墨水. 这种技术能够精确,远程控制工程组织中的基因转染,从而推进疾病建模和再生医学.

科学领域:

生物技术是生物技术.
再生医学是一种再生医学.
生物材料是一种生物材料.

背景情况:

同轴3D生物打印为疾病建模和治疗创造了先进的组织结构.
由于载体扩散有限,控制3D支架中的基因表达具有挑战性.
现有的转染方法在密集的3D生物打印结构中缺乏时空控制.

研究的目的:

开发一种具有受控基因传递能力的3D生物打印技术.
为了克服传统转化方法在密集的3D支架中的局限性.
在工程组织中实现时空定义的基因操纵.

主要方法:

开发了使用超声响应基因传递生物墨水的同轴挤出3D生物打印.
包含含有DNA有效载荷的脂涂层微气泡,用于载有细胞的酸生物墨水.
利用聚焦超声波来诱导声波和促进DNA输送到生物打印结构中的细胞.

主要成果:

已建立的同轴打印参数保持粒子稳定性和高细胞活力48小时.
取得了成功的超声波,DNA传递和超声波控制的转基因表达.
通过调整超声波脉冲和微泡度来证明转染细胞数量和输送区域的调节.

结论:

介绍了一种成功的同轴3D生物打印技术,用于超声波控制的基因传递.
这个平台允许在生物打印组织结构中进行远程,空间时间定义的基因操纵.
该技术在疾病建模和再生医学方面有着重要的应用.

关键词:

生物墨水是生物墨水.生物材料是一种生物材料.同轴3D生物打印技术有控制的交付方式.聚焦式超声波的使用方法基因输送基因输送基因输送微气泡是一种微气泡.通过超声波检测进行了超声波检测.超声波超声波是指超声波的使用.

更多相关视频

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Published on: November 18, 2016

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Published on: January 3, 2018

相关概念视频

Recombinant DNA

Recombinant DNA

Transgenic Organisms

Transgenic Organisms

Types of Genetic Transfer Between Organisms

Types of Genetic Transfer Between Organisms

Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.

Recombinant DNA

Recombinant DNA

Transgenic Organisms

Transgenic Organisms

Types of Genetic Transfer Between Organisms

Types of Genetic Transfer Between Organisms

Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.