Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Recent Advances in Molecular Engineering of Organelle-Targeted Viscosity Probes for Fluorescence and Biological Applications.

ACS sensors·2026
Same author

Laparoscopic versus open pancreaticoduodenectomy for non-pancreatic periampullary adenocarcinomas: study protocol for a multicentre randomised controlled trial in China.

BMJ open·2026
Same author

Optimizing nitrogen management enhances photosynthesis and alfalfa productivity in jujube-alfalfa intercropping.

Scientific reports·2026
Same author

Diagnostic Performance of Serum AKR1B10 in Early-Stage and AFP-Negative Hepatocellular Carcinoma: A Multicentre Study.

Liver international : official journal of the International Association for the Study of the Liver·2026
Same author

Evidence supporting the role of GIGYF2 in synapse development and autism.

Molecular psychiatry·2026
Same author

Fusion prediction model for post-ERCP pancreatitis under NSAIDs prophylaxis.

Surgical endoscopy·2026

相关实验视频

Updated: Jun 8, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

17.5K

莫比奇普 (MobiChIP):一种基于单细胞奇普-seq的滴滴的兼容图书馆构建方法.

Xianhong Yu1,2, Guantao Zheng2, Liting Xu3

  • 1The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics and Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.

Molecular omics
|November 8, 2024
PubMed
概括

MobiChIP是一种新的单细胞ChIP-seq方法,用于准确地绘制表观遗传异质性的地图. 它有效地捕获染色体碎片并揭示调节景观,在基因抑制分析中表现优于ATAC-seq.

更多相关视频

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K

相关实验视频

Last Updated: Jun 8, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

17.5K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K

科学领域:

  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 基因组学就是基因组学.
  • 分子生物学分子生物学

背景情况:

  • 单细胞表观遗传学对于理解细胞异质性至关重要.
  • 现有的单细胞ChIP-seq (scChIP-seq) 方法需要优化,以提高准确性和方便性.
  • 在单细胞分辨率下绘制基因基因修饰的图表对于破译基因调节至关重要.

研究的目的:

  • 开发一种多功能且准确的scChIP-seq库构建方法.
  • 证明新方法用于分析跨物种和组织的表观遗传异质性的实用性.
  • 将新方法的性能与现有技术 (如ATAC-seq.) 进行比较.

主要方法:

  • 开发了MobiChIP,一种基于标记的ChIP-seq库准备方法,用于单细胞应用程序.
  • 将MobiChIP应用于外周血液单核细胞 (PBMCs),以分析活性 (H3K27ac) 和抑制性 (H3K27me3) 基因组修饰.
  • 集成的MobiChIP与单细胞RNA测序 (scRNA-seq) 进行多原子分析.
  • 将MobiChIP性能与ATAC-seq进行比较,以识别表观遗传抑制.

主要成果:

  • MobiChIP有效地捕获来自不同物种的标记核的染色质碎片.
  • 该方法允许从不同的组织或物种进行样本复合.
  • 在PBMC中,MobiChIP精确地确定了Hox基因集群的表观遗传抑制,其表现优于ATAC-seq.
  • 强大的核细胞扩增和灵活的测序可以在没有定制的原始体的情况下实现.
  • 综合的scChIP-seq和scRNA-seq揭示了综合的遗传和表观遗传异质性.

结论:

  • MobiChIP提供了一个强大的,灵活的,准确的工具,用于单细胞ChIP-seq分析.
  • 这种方法在单细胞分辨率下增强了对表观遗传异质性和监管景观的研究.
  • 对于特定的表观遗传分析,例如基因抑制映射,MobiChIP比ATAC-seq具有优势.