相关实验视频

Updated: May 7, 2026

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease

Published on: April 12, 2021

血管化人类肺部器官体的生理模型

Abdul S Qadir^1,2, Sukanta Das^1,2, Swathi Nedunchezian^1,2

¹Department of Pharmacology and Regenerative Medicine.

American journal of respiratory cell and molecular biology

|November 8, 2024

概括

使用诱导的多能干干细胞和内皮细胞创建了血管化的人类肺器官 (hLO). 这些有机体模仿肺部发育和炎症反应,为研究肺部疾病提供了一个新的模型.

科学领域:

再生医学是一种再生医学.
发展生物学发展生物学
干细胞研究干细胞研究

背景情况:

来自诱导多能干细胞 (iPSCs) 的人类肺器官 (hLOs) 模型肺发育.
血管化在hLO中对改善表现的作用尚不清楚.

研究的目的:

通过将内皮细胞 (ECs) 与iPSC衍生的肺前代细胞 (LPs) 集成来开发和表征血管化的hLO.
评估血管化对hLO分化和炎症反应的影响.

主要方法:

通过将人类iPSC衍生的LP与EC结合在不同比例下生成的hLO.
在NOD/SCID小鼠的囊中植入血管化的hLO用于体内评估.
使用包括电子显微镜和LPS挑战在内的技术分析了膜表皮亚型 (ATII和ATI细胞) 和炎症反应的分化.

主要成果:

最佳的LP:EC比率导致了hLO内集成的血管,在体内观察到红细胞.
血管化的hLO显示出ATII和ATI细胞,表面活性剂的产生和原始的气道结构.
移植的血管化hLOs对LPS挑战产生了显著的炎症反应,其中有中性粒细胞的涌入.

结论:

hLO的血管化增强了它们对肺部发育的表现,包括上皮分化和炎症反应.
血管化的hLO作为研究人类肺部发育和炎症性肺损伤的有希望的模型.
需要进一步的研究才能达到成熟的人类肺膜上皮细胞比率 (1:9 ATII/ATI).

关键词:

膜上皮细胞膜上皮细胞它们是内皮细胞的内皮细胞.诱导的多能干细胞干细胞.这是一种炎症炎症炎症炎症.血管化的人类肺部器官.

更多相关视频

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

Published on: March 23, 2022

Author Spotlight: Improving Reproducibility in Vascular Organoids Using ROCK Inhibitors and Microwell Confinement

Author Spotlight: Improving Reproducibility in Vascular Organoids Using ROCK Inhibitors and Microwell Confinement

Published on: December 13, 2024

相关概念视频

Gross Anatomy of the Lungs

Gross Anatomy of the Lungs

The lungs are a pair of vital organs connected to the trachea via the left and right bronchi. The base of these organs meets the dome-shaped muscle known as the diaphragm. Encased by the pleurae, the lungs contact the mediastinum. The right lung is shorter yet wider, and has a larger volume than the left lung. The left lung has an indentation known as the cardiac notch. The superior region of the lungs is referred to as the apex, whereas the base is the lower region near the diaphragm. The...