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多频STD核磁共振揭示了来自Ruminococcus gnavus的第一个分子内跨酸酶的迈凯利斯复合体

Serena Monaco¹, Louise E Tailford², Andrew Bell²

¹School of Chemistry, Pharmacy & Pharmacology, University of East Anglia, Norwich Research Park, NR4 7TJ Norwich, UK.

Bioorganic chemistry

|November 8, 2024

概括

鲁米诺科克斯 (Ruminococcus gnavus) 表达RgNanH,一种分子内跨酸酶,以分解粘膜. 结构研究揭示了它的α-2,3特异性,对肠道细菌的新陈代谢和繁殖至关重要.

科学领域:

微生物学微生物学
结构生物学结构生物学
葡萄糖生物学葡萄糖生物学

背景情况:

RgNanH是一种来自肠道细菌Ruminococcus gnavus的内分子转酸酶.
它利用人类肠道环境中的化粘素甘氨酸.
RgNanH属于甘氨酸酸酶GH33家族,并分裂酸.

研究的目的:

为了阐明RgNanH对sialoglycans的特异性,动力学和亲和力.
提出了第一个三维模型的内分子转酸酶迈凯利斯复合体.
了解RgNanH酶活性的分子机制.

主要方法:

和转移差异核磁共振 (STD NMR).
计算技术包括分子对接和CORCEMA-ST.
用于模型验证的差异性皮层映射STD NMR (DEEP-STD NMR).

主要成果:

作为主要的识别元素,RgNanH对酸具有特异性.
确定了Trp698在CH-π与3'-sialyllactose的银河糖残留物堆叠中的关键作用.
RgNanH显示α-2,3特异性,使其与非基质6'-sialyllactose区分开来.
迈凯利斯复合体的3D模型被提出并在解决方案中验证.

结论:

这项研究提供了第一个关于分子内跨酸酶迈凯利斯复合物的结构性见解.
Trp698对于观察到的RgNanH的α-2,3特异性至关重要.
像STD NMR这样的结构方法对于研究没有X射线结构的酶机制是有价值的.

关键词:

CH-π 相互作用在DEEP-STD的NMR指纹中,停靠计算对接计算肠道微生物群与肌的相互作用内部分子的跨化酶.多频STD的NMR是一种多频STD.蛋白质与碳水化合物的相互作用

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