通过Bacillus natto在氨运动和土壤水过程中的固态发酵来生产聚γ-胺
Xin Wang1, Jie Gao2, Jiahui Wu1
1School of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, Shandong 266042, China.
Enzyme and microbial technology
|November 8, 2024
概括
研究人员开发了一种高聚聚胺酸 (γ-PGA) 产生的Bacillus natto菌株. 由此产生的固体发酵产品 (SFP) 有效地净化水,改善土壤的保留水和植物生长.
科学领域:
- 微生物学和生物技术
- 环境科学 环境科学
- 农业科学 农业科学
背景情况:
- 聚聚胺酸 (γ-PGA) 是一种具有多种应用的可生物降解聚合物.
- 优化γ-PGA的微生物生产对于其工业可行性至关重要.
- 微生物发酵产品的环境应用需要进一步研究.
研究的目的:
- 使用突变发生学来选和识别高γ-PGA产生的Bacillus natto菌株.
- 确定 γ-PGA 生产的最佳固态发酵 (SSF) 条件.
- 评估γ-PGA固体发酵产品 (SFP) 的环境和农业应用.
主要方法:
- 紫外线突变性被用来增强Bacillus natto.中的γ-PGA生产.
- 使用单向差异分析来优化SSF的发酵介质组成.
- 用了特征化技术来确认γ-PGA的存在和分子量.
- 进行了水净化,土壤保留水和植物生长试验.
主要成果:
- 一种高γ-PGA产生Bacillus natto菌株 (UV-40-50) 已成功开发.
- 在最佳的SSF条件下,产量为55.19g/kg的γ-PGA.
- SFP在从水中去除氨 (≥60%) 和酸盐 (≥40%) 中显示出显著的有效性.
- SFP的应用增强了土壤的保留水 (菜土:2.13倍,生菜土:12%) 和改善了生长率和生菜和大白菜的苗木长度.
结论:
- 开发的Bacillus natto菌株是高γ-PGA生产的有希望的来源.
- 来自该菌株的SFP表现出强大的净水能力.
- SFP显著改善了土壤的保留水,并促进了植物生长,表明其作为可持续农业修正案的潜力.
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