反氧介导的dsDNA染色光氧化酶模仿使得3,3',5,5'-四甲基二因在中性pH下通过溶解的O2进行催化氧化,以改善生物感知
Peng'an Zhu1, Shuang Liu1, Lijun Feng1
1College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu, 610059, China.
Biosensors & bioelectronics
|November 8, 2024
概括
一种新的DNA染色光氧化酶模拟器使用单片氧来氧化中性pH的TMB. 该系统为生物分子和重金属的敏感生物传感提供可调节的活动.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
背景情况:
- 在中性pH下对3,3',5,5'-四甲基 (TMB) 的催化氧化对于放大生物分析至关重要.
- 现有的纳米酶在中性pH下对同质生物分子检测进行调节活动而扎.
研究的目的:
- 开发一种以氧化还原为媒介的DNA染料光氧化酶模仿剂,用于TMB的催化氧化,在中性pH下通过溶解的O2进行催化氧化.
- 实现模块化活动,以改善均生物传感应用.
主要方法:
- 使用双链DNA-SYBR绿色I (dsDNA-SG) 系统在照明下进行单片氧 (1O2) 的光生成.
- 采用Mn3+/Mn2+氧化还原介质,以在中性pH下有效的TMB氧化.
- 研究了通过dsDNA形成/解离来调节催化活性.
主要成果:
- 在pH 7.0下,在Mn2+介导的dsDNA-SG光氧化酶模拟剂的最大反应速率为1.7 × 10-4 mM/s,超过了胡卜过氧化酶 (HRP).
- 与pH值4.0相比,在pH值7.0下APE1检测的灵敏度超过130倍,比pH值4.0更高.
- 扩展了在中性pH下敏感检测DNA (LOD,8 pM) 和重金属离子的系统.
结论:
- 氧化还原介导的dsDNA-dye光氧化酶模拟有效地在中性pH下催化TMB氧化.
- 该系统可调节的酶活性和中性pH运行使其非常适合各种生物传感应用.
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