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相关概念视频

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Semiconductors01:22

Semiconductors

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There is variation in the electrical conductivity of materials - metals, semiconductors, and insulators that are showcased with the help of the energy band diagrams.
Metals such as copper (Cu), zinc (Zn), or lead (Pb) have low resistivity and feature conduction bands that are either not fully occupied or overlap with the valence band, making a bandgap non-existent. This allows electrons in the highest energy levels of the valence band to easily transition to the conduction band upon gaining...
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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Integrator and Differentiator01:13

Integrator and Differentiator

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Op-amp circuits have significant applications in various fields, including automotive engineering. One such application is cruise control systems in cars, where op-amp circuits are integral for maintaining a constant speed. In these systems, op-amps function as both integrators and differentiators.
An integrator within an op-amp circuit produces an output directly proportional to the integral of the input signal. This is achieved by replacing the feedback resistor in a typical inverting...
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Block Diagram Reduction01:22

Block Diagram Reduction

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The process of deriving the transfer function of a control system often involves reducing its block diagram to a single block. This simplification can be achieved through a series of strategic operations, including relocating branch points and comparators. These operations preserve the overall function of the system while allowing for easier manipulation and combination of blocks.
The first step in this process is the identification and relocation of a branch point. A branch point, where a...
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Circuit Terminology01:14

Circuit Terminology

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An electrical network is a system composed of interconnected elements, such as resistors, capacitors, inductors, and voltage or current sources. Unlike a circuit, an electrical network does not necessarily form a closed path. In other words, while all circuits can be considered networks due to their interconnected nature, not every network qualifies as a circuit.
A circuit, on the other hand, is also an interconnected system of electrical elements but must contain one or more closed paths.
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相关实验视频

Updated: Jun 8, 2025

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
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Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

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微处理器和一个变体NEXT复合体之间的功能连接

Katsutoshi Imamura1, William Garland2, Manfred Schmid2

  • 1Department of Molecular Biology and Genetics, Universitetsbyen 81, Aarhus University, Aarhus, Denmark; Department of Systems Medicine, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.

Molecular cell
|November 8, 2024
PubMed
概括
此摘要是机器生成的。

微处理器复合体处理初级miRNA,其DGCR8蛋白与NEXT复合体进行链接. 该合作伙伴关系针对小鼠胚胎干细胞中的特定RNA结构,揭示了一种新的RNA降解途径.

关键词:
总局CR8 总局CR8 总局微处理器微处理器下一个复杂的复杂的NEXT.结构 RNA 结构 RNA 结构在 ZCCHC8 中.核RNA衰变中的核RNA衰变

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相关实验视频

Last Updated: Jun 8, 2025

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

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科学领域:

  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.
  • 基因规则 基因规则

背景情况:

  • 主要的microRNAs (miRNAs) 是由哺乳动物细胞中的微处理器复合体 (DROSHA-DGCR8) 处理的.
  • RNA外基因组途径降解各种RNA基质,但其特定的向机制仍在被阐明.

研究的目的:

  • 为了研究微处理器对初级miRNAs的裂变后产生的5'侧边区域的命运.
  • 为了识别参与这些RNA片段和其他结构RNA的周转的蛋白质复合体.

主要方法:

  • 生物化学测试用于研究蛋白质-RNA相互作用.
  • 突变性研究探讨特定蛋白质域的功能.
  • 在小鼠胚胎干细胞 (mESCs) 中对RNA处理和降解的分析.

主要成果:

  • 核外体向 (NEXT) 综合体,特别是它的ZCCHC8组件,与DGCR8进行物理相互作用.
  • 一种包括MTR4和ZCCHC8但缺少RBM7的NEXT复合体的变体,负责准含有RNA的茎环,包括那些来自微处理器活动的RNA.
  • 这种MTR4-ZCCHC8复合体还向其他结构RNA,例如增强剂的RNA,证明了基质的灵活性.

结论:

  • MTR4-ZCCHC8 NEXT复合体与微处理器 (DGCR8 / DROSHA) 连接,以降解特定的RNA结构.
  • RNA结构上下文决定了包含RBM7或DGCR8链接的NEXT复合体的向,揭示了复杂的RNA监控机制.