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Updated: Jun 8, 2025

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从诱导的多能干细胞干细胞中衍生可移植的人类甲状腺毛囊上皮细胞

Hendrik J Undeutsch¹, Alberto Posabella², Andrea B Alber³

¹Division of Endocrinology, Diabetes and Metabolism, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medicine, New York, NY, USA; Department of Medicine, Boston University Chobanian and Avedisian School of Medicine and Boston Medical Center, Boston, MA, USA; Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA, USA.

Stem cell reports

|November 8, 2024

概括

研究人员开发了一种新的方法,从人类诱导的多能干细胞中产生甲状腺毛囊细胞. 这些细胞对甲状腺功能低下症的治疗有前途,但未能在小鼠中完全恢复甲状腺功能.

科学领域:

干细胞生物学干细胞生物学
内分泌学在内分泌学.
发展生物学发展生物学

背景情况:

甲状腺毛囊细胞 (TFCs) 对于治疗甲状腺功能低下症至关重要.
从人类诱导的多能干细胞 (iPSC) 产生功能性TFC是一个关键的治疗目标.

研究的目的:

开发一种方法,从iPSC中生产成熟的,功能性的TFC.
在体内评估iPSC衍生的TFCs的治疗潜力.

主要方法:

开发了一种新型的iPSC线与NKX2-1和PAX8位点光体用于细胞识别和净化.
优化了使用骨形态蛋白4 (BMP4) 和纤维细胞生长因子2 (FGF2) 的逐步无血清分化协议.
在免疫缺陷小鼠中利用单细胞RNA测序和异种移植.

主要成果:

成功地将iPSC分化为表达特定标记物的TFC (NKX2-1,PAX8).
在一个月内,BMP4的退出促进了TFC的成熟.
异种移植的细胞形成了表达甲状腺蛋白的甲状腺毛囊结构.
从iPSC衍生的TFC长期表达甲状腺特异性蛋白质,但没有在体内拯救甲状腺功能低下症.

结论:

一个新的协议有效地从具有适当的毛囊组织和蛋白质表达的iPSC产生TFC.
虽然有前途,但iPSC衍生的TFC目前缺乏在体内治疗甲状腺功能低下症的全部功能能力.

关键词:

有针对性的差异化.人类人类人类人类人类人类人类有机生物有机物多能干细胞是一种多能干细胞.甲状腺是什么?甲状腺是什么?

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