卡尔西醇通过降低ACE2和TMPRSS2表达的调节,可以预防人体热囊细胞中SARS-CoV尖端诱导的炎症
Rafael Vargas-Castro1, Janice García-Quiroz1, Andrea Olmos-Ortiz2
1Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Ciudad de México, Mexico.
维生素D () 减少了SARS-CoV-2尖端蛋白在胎盘细胞中的炎症作用. 它降低了renin-angiotensin系统的关键组成部分和TMPRSS2,可能限制病毒感染,并在怀孕期间调节血压.
科学领域:
- 生殖生物学 生殖生物学
- 病毒学 病毒学
- 内分泌学 在内分泌学.
背景情况:
- 感染SARS-CoV-2会导致妊娠并发症的风险,例如高血压疾病和胎盘炎症.
- 病毒利用尖端糖蛋白通过ACE2和TMPRSS2进入细胞,影响调节血压的氨酸- ангиотензин系统 (RAS).
- 众所周知,维生素D,特别是酸,可以调节RAS,炎症和免疫力,但其在对SARS-CoV-2的胎盘反应中的作用尚不清楚.
研究的目的:
- 为了研究酸醇对SARS-CoV-2尖端诱导炎症和人类胎盘细胞中的RAS成分的影响.
- 为了确定酸醇是否影响胎盘中ACE1,ACE2和TMPRSS2的表达.
- 探索酸醇对ACE1/ACE2比率及其对胎盘细胞的抗炎作用的影响.
主要方法:
- 利用了HTR-8/SVneo trofhoblast细胞系和初级合成性热细胞.
- 使用RT-qPCR测量基因表达水平.
- 应用ELISA用于细胞因子分析和免疫细胞化学用于蛋白质定位.
主要成果:
- 在SARS-CoV-2中,尖端蛋白显著增加了胎盘细胞中的促炎性细胞因子.
- 醇治疗显著降低了这些炎症性细胞因子的表达和分泌.
- 醇降低了ACE1,ACE2,TMPRSS2和雷宁的基因表达,并降低了ACE1/ACE2mRNA比率.
结论:
- 酸有效地通过减少关键RAS成分和TMPRSS2.2的表达来抑制人类胎盘细胞中尖端诱导的炎症.
- 维生素D在限制SARS-CoV-2的胎盘感染方面发挥作用,通过使热囊细胞不那么容易受到感染.
- 酸可以帮助调节孕妇的血压,减少炎症,在怀孕期间提供保护作用.
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