Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Ras Gene02:38

The Ras Gene

6.2K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.2K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

6.4K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.4K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

3.9K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
3.9K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

3.4K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.4K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

8.7K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Spatially Organized Human Ovarian Spheroids Instruct Endometrial Morphogenesis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Bioengineering Ovarian Endocrine Function: From Follicle Modeling to Cell-Based Hormone Therapy.

Advanced healthcare materials·2026
Same author

A 25+ Year Journey on Yeast-Regulated Cell Death Research.

Current topics in microbiology and immunology·2026
Same author

Effect of Aromatherapy Massage With <i>Foeniculum vulgare</i> Mill. Seed Essential Oil Compared to Massage on Anxiety, Well-Being, and Sleep Quality: An Exploratory Randomized Study.

Health science reports·2026
Same author

Breaking free: Lung herniation following epicardial lead placement.

Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation·2026
Same author

Nitrogen utilization diversity in Torulaspora delbrueckii isolates relevant to food and beverage fermentations.

Food microbiology·2026

相关实验视频

Updated: Jun 12, 2025

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
07:49

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Published on: July 17, 2019

6.0K

瘤性KRAS突变调节BAX介导的细胞死亡.

Anabela Ferreira1, Stéphen Manon2, Akandé Rouchidane Eyitayo2

  • 1Centre of Molecular and Environmental Biology (CBMA), Department of Biology, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal; Institute of Science and Innovation for Bio-Sustainability (IB-S), Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal.

Biochimica et biophysica acta. Molecular cell research
|November 8, 2024
PubMed
概括

在结肠直肠癌 (CRC) 中,突变的基尔斯顿大鼠肉瘤病毒瘤基因同源 (KRAS) 通过直接与亲亡蛋白 BAX.相互作用,赋予细胞死亡的抵抗力. 这种相互作用会影响BAX诱导的细胞死亡和细胞染色体c的释放.

关键词:
巴克斯巴克斯 (BAX) 是一个结肠直肠癌是一种癌症.人性化的酵母酵母克拉斯 (Kras) 是一个国家.致癌突变 致癌突变 致癌突变

更多相关视频

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
10:09

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Published on: January 7, 2019

8.2K
Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

17.9K

相关实验视频

Last Updated: Jun 12, 2025

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
07:49

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Published on: July 17, 2019

6.0K
Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
10:09

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Published on: January 7, 2019

8.2K
Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

17.9K

科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 在瘤学瘤学.

背景情况:

  • 基尔斯大鼠肉瘤病毒瘤基因同源 (KRAS) 是一种参与细胞信号传递和细胞亡的GTPase.
  • 瘤性KRAS突变在30-50%的结直肠癌 (CRC) 患者中普遍存在.
  • 克拉斯突变在细胞亡调节中的作用,特别是关于BAX的作用,尚不清楚.

研究的目的:

  • 调查人类突变KRAS在调节BAX中的作用,BAX是一种关键的亲亡蛋白.
  • 为了阐明KRAS异型和BAX之间的相互作用.
  • 在KRAS突变和BAX的背景下,探索酸对细胞死亡的影响.

主要方法:

  • 使用了酵母模型Saccharomyces cerevisiae.
  • 同表达的野生类型KRAS (KRASWT) 或常见的CRC KRAS突变 (KRASG12D,KRASG12V,KRASG13D) 与缺乏ras2异型的酵母细胞中的人类BAX.
  • 评估细胞死亡,细胞染色体c的释放,以及蛋白质在线粒体的同定位.

主要成果:

  • 与KRASWT不同的是,KRAS突变对BAX诱导的细胞死亡和细胞染色体c释放产生了抵抗力.
  • 证据表明,KRAS异型和BAX.之间的直接相互作用和线粒体同定位.
  • 乙酸显著增加了表达BAX和瘤性KRAS突变细胞的细胞死亡,但不是KRASWT.

结论:

  • 突变的KRAS直接调节BAX功能,导致对亡的抵抗.
  • 这些发现表明KRAS和BAX在线粒体之间存在直接的物理相互作用.
  • 瘤性KRAS突变可能使癌细胞对酸盐敏感,提供潜在的治疗见解.