相关实验视频

Updated: Jun 8, 2025

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
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Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

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一种简单且具有成本效益的实时PCR方法,使用稀释和热处理全血溶解酸

Gökçe Güllü Amuran1, Büşra Polat2, Abdülkadir Kahraman2

  • 1Department of Medical Biology, School of Medicine, Marmara University, Başıbüyük Mah. Maltepe Başıbüyük Yolu Sok. No:9/2, Istanbul, 34899, Turkey. gokcegullu@gmail.com.

Scientific reports
|November 8, 2024
PubMed
概括

这项研究引入了一种新的"GG-RT PCR"方法,该方法绕过DNA提取,使用血液溶解物进行实时PCR. 这种具有成本效益的技术简化了用于遗传分析的样本准备.

关键词:
DNA 隔离 DNA 隔离直接进行PCR.直接实时PCR进行实时PCR.方法 方法 方法 方法实时PCR可以实现.

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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