基于REIMS结合二维LC-MS的二维LC-MS的野生和栽培阿斯特拉加利根的识别策略
Sijian Chen1, Xiaoshuang Li1, Danshu Shi2
1State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
NPJ science of food
|November 8, 2024
概括
使用快速蒸发性电离质谱法 (REIMS) 和2D-LC-MS的新方法可以区分野生和栽培的阿斯特拉加利. 这种技术为传统中医分析提供了快速的实时识别.
科学领域:
- 分析化学 分析化学
- 药理学是指药理学,即药理学是指药理学.
- 传统中国医学 (TCM) 是一种
背景情况:
- 准确识别药用草药,如阿斯特拉加利草根,对于中国传统医学 (TCM) 的质量控制至关重要.
- 由于表型变异,区分野生 (WAR) 和栽培 (CAR) 的阿斯特拉加利根茎具有挑战性.
- 现有的草药识别方法可能耗时,需要大量的样本准备.
研究的目的:
- 建立一个快速和实时的方法来区分野生阿斯特拉加利根 (WAR) 和培养的AR (CAR).
- 为了全面描述和量化WAR和CAR中的关键化合物.
- 为TCM识别和质量保证提供可靠的分析方法.
主要方法:
- 结合快速蒸发性电离质谱 (REIMS) 与二维液体染色学-质谱 (2DLC-MS) 进行环境电离分析.
- 开发了一个基于WAR和CAR的表型数据的实时识别模型.
- 利用超高性能液态色谱/四极线性离子陷质谱 (UHPLC-QTRAP-MS) 来对45种已识别的化合物进行半定量分析.
主要成果:
- 该REIMS和2DLC-MS方法使得快速的实时识别,没有复杂的样本准备.
- 多变量统计分析确定了WAR和CAR之间的45种差异性化合物.
- 发现大多数化合物的相对含量在WAR中比CAR更高.
结论:
- 开发的方法有效地区分了野生和栽培的Astragali Radix.
- 这种方法为TCM的质量控制和识别提供了有价值的工具.
- 这些发现有助于确保Astragali Radix在药物应用中的真实性和有效性.
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