相关实验视频

Updated: Jun 8, 2025

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

缺氧诱导因子异构和免疫检查点调节器在清细胞细胞癌中的空间功能映射

Elena Safrygina^1,2, Christopher Applebee¹, Alan McIntyre³

¹Cell Biophysics Laboratory, Centre for Therapeutic Innovation, Life Science Department, University of Bath, Claverton Down, Bath, BA2 7AY, UK.

|November 8, 2024

概括

一种新的计算方法,FuncOmap,量化了HIF2α在清细胞细胞癌 (ccRCC) 的功能状态. 这改善了针对性治疗的患者分层,超越了简单的表达水平.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物物理学的生物物理.

背景情况:

清细胞细胞癌 (ccRCC) 是一种致命的癌亚型.
在ccRCC中VHL突变稳定HIF1α和HIF2α,影响癌症的进展.
评估HIF2α激活,而不仅仅是表达,对于预测治疗反应至关重要.

研究的目的:

开发一种新的计算方法,功能瘤学地图 (FuncOmap),用于评估HIF2α激活状态.
改进ccRCC中针对性治疗的患者分层.
提高功能性蛋白质组学的临床可访问性.

主要方法:

利用两位点放大时间解析的福斯特共振能量转移 (aiFRET) 进行功能蛋白质组学量化.
开发了FuncOmap,这是一个用于绘制和量化蛋白功能状态的计算工具.
应用FuncOmap来分析ccRCC患者样本中的HIF2α和HIF1β的空间相互作用状态.

主要成果:

在高空间分辨率下,FuncOmap可直接绘制和量化蛋白功能状态.
FuncOmap集成了表达和功能状态成像,用于共同分析.
证明了在ccRCC中可视化HIF2α和HIF1β的空间交互状态的能力.

结论:

FuncOmap准确量化了患者反应异质性的数量.
这种方法通过改善患者分层来提高精准医学.
通过评估功能生物标志物,FuncOmap促进了针对性疗法的最佳临床使用.

更多相关视频

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...