相关实验视频

Updated: Jun 7, 2025

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

研究使用循环瘤DNA用于肉瘤管理的研究

Paige Darville-O'Quinn¹, Nalan Gokgoz¹, Kim M Tsoi^1,2,3

¹Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON M5G 1X5, Canada.

Journal of clinical medicine

|November 9, 2024

概括

在肉瘤患者中检测循环瘤DNA (ctDNA).

科学领域:

在瘤学瘤学.
分子诊断学分子诊断
基因组学就是基因组学.

背景情况:

瘤是一种具有高复发率和转移率的侵袭性癌症.
缺乏早期诊断生物标志物使得及时治疗变得复杂.
需要方法来监测疾病的进展和治疗反应.

研究的目的:

在肉瘤患者的血中调查ctDNA检测.
开发个性化测试以监测疾病复发和进展.
评估敏感的方法来检测少量的ctDNA.

主要方法:

瘤和血液样本的全外体测序以确定患者特定的突变.
开发用于ctDNA检测的个性化检测方法.
滴滴数字PCR (ddPCR) 和多重PCR与安普利康测序的比较.

主要成果:

使用ddPCR成功检测了瘤特异性血突变,证实了ctDNA的存在.
多重PCR用安普利康测序检测到多个变异,但比ddPCR敏感度低.
在不同时间点的血样本中检测到ctDNA.

结论:

个性化ctDNA测试显示了监测肉瘤进展的潜力.
解决了在肉瘤管理中对生物标志物的需求.
突出了ctDNA在临床瘤学环境中的有用性.

关键词:

生物标志物生物标志物没有细胞的DNA DNA.在循环中的瘤DNA.ctDNActDNA是什么意思 ddPCRR 在线播放液体活检活检液体活检多重复合PCR是一种多重复合PCR.肉瘤肉瘤是一种肉瘤.软组织肉瘤软组织肉瘤

更多相关视频

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

Published on: June 13, 2019

相关概念视频

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...