与血液学参数和T淋巴细胞亚种群相关的基因组变异在大型白猪和小猪交叉种群中的T淋巴细胞亚种群
Naiqi Niu1, Runze Zhao1, Ming Tian2
1State Key Laboratory of Animal Biotech Breeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China.
Animals : an open access journal from MDPI
|November 9, 2024
概括
这项研究使用全基因组关联来识别猪血特征的遗传标记,帮助疾病耐药性繁殖和人类疾病研究. 确定了影响免疫细胞计数和血小板特征的关键基因.
科学领域:
- 动物遗传学动物遗传学
- 免疫学 免疫学 免疫学
- 进行比较基因组学.
背景情况:
- 抵抗疾病的养猪对猪业至关重要.
- 猪血指标为动物健康和人类疾病提供了洞察力.
- 影响血液特征的遗传因素对于改善抗病能力至关重要.
研究的目的:
- 在猪中识别与17个血液学和T淋巴细胞参数相关的遗传变异.
- 发现影响免疫细胞亚群和血小板特征的候选基因.
- 为养殖抗病猪提供遗传基础,了解相关的人类状况.
主要方法:
- 从大白猪和小猪产生的F2资源种群 (489只)
- 测量了17个血液学参数和T淋巴细胞亚群.
- 使用Illumina PorcineSNP60 BeadChip和TASSEL 5.0软件进行了全基因组关联研究 (GWAS).
主要成果:
- 在SSC7上确定了血小板计数 (PLT) 和百分比 (PCT) 的显著SNP位点,并在SSC12上确定了白细胞计数 (WBC).
- 在SSC7上检测到T淋巴细胞亚群 (例如CD4+/CD8+) 的显著关联,确定了候选基因CLIC5,TRIM15和SLC17A4.
- 在SLC17A4,TRIM15和CLIC5中发现了特定的误解变异,与免疫细胞特征有显著的关联. 确定了作为关键途径的MHCII类蛋白质复合体结合.
结论:
- 全基因组关联映射成功确定了猪血特征的遗传位置和候选基因.
- 像SLC17A4,TRIM15和CLIC5这样的特定基因在调节免疫细胞种群方面发挥着作用.
- 研究结果支持在生殖抗病猪中使用标记器辅助选择的可能性,并为人类健康研究提供了比较的见解.
关键词:
在CD4+/CD8+中使用.CD4 - CD8 + CD3 + CD3 + CD4 + CD8 + CD3 + CD4 + CD8 + CD3 + CD8 + CD3 + CD4 + CD8 + CD8 + CD3 + CD3 + CD4 + CD8 + CD4 + CD8 + CD8 + CD3 + CD3 + CD4 + CD8 + CD4 + CD8 + CD3 + CD8 + CD9 + CD9 + CD9 + CD9 + CD9 + CD9 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 + CD10 +在SNP中,SNP是SNP.全基因组关联研究研究.血小板计数 血小板计数 血小板计数 血小板计数相关概念视频
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