评估乳制品农场内的 Clostridioides difficile及其毒素传播风险
Valentina A Filippova1,2, Larisa A Ilina1,2, Elena A Yildirim1,2
1Department of Large Livestock Husbandry, St. Petersburg State Agrarian University, St. Petersburg-Pushkin 196605, Russia.
Animals : an open access journal from MDPI
|November 9, 2024
概括
在奶牛料中发现了Clostridioides difficile毒素,这表明它是感染的来源. 患病的动物分泌更多的毒素,这表明疾病易感性与病原体存在有关.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 微生物学 微生物学
- 动物科学动物科学
背景情况:
- 困难菌是一种重要的胃肠道病原体,影响人类和动物的健康.
- 由于C. difficile的流行率越来越高,因此需要了解其传播途径和对牲畜的影响.
研究的目的:
- 为了调查在牛场的Clostridioides difficile毒素的存在和来源.
- 分析奶牛的便微生物组变化及其与C. difficile感染和生产率的相关性.
主要方法:
- 用PCR检测在料和便样本中的C. difficile毒素.
- 下一代测序 (NGS) 分析了奶牛料和便的微生物组合.
- 从各种农场位置和动物健康状况收集样本.
主要成果:
- 在料样本中检测到C. difficile毒素A和二进制毒素基因.
- 患病动物的便显示,与健康动物相比,C. difficile毒素基因的患病率是健康动物的2-3倍.
- 在一个月的时间里,观察到便微生物组结构的显著变化,包括病原细菌和患病动物中的甲原古生物的增加.
结论:
- 料似乎是C. difficile进入牛胃肠道的可能来源.
- 削弱的动物,特别是那些患有现有疾病的动物,更容易受到C. difficile的殖民和相关的生产力损失的影响.
- 便微生物组的改变可能表明受影响的牛的料效率和动物生产率下降.
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