在自然杀手T细胞上缺乏甲状腺刺激激素受体表达:对免疫内分泌相互作用的影响
Emilia Adamska-Fita1, Przemysław Wiktor Śliwka1,2, Małgorzata Karbownik-Lewińska1,2
1Department of Endocrinology and Metabolic Diseases, Polish Mother's Memorial Hospital-Research Institute, 93-338 Lodz, Poland.
International journal of molecular sciences
|November 9, 2024
概括
甲状腺刺激激素受体 (TSHR) 在自然杀手T (NKT) 细胞上没有表达,尽管它存在于其他免疫细胞上. 这一发现挑战了NKT细胞在自身免疫性甲状腺疾病发展中的直接作用.
科学领域:
- 免疫学 免疫学 免疫学
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
背景情况:
- 甲状腺刺激激素受体 (TSHR) 的表达已知存在于各种免疫细胞上,包括B和T淋巴细胞,NK细胞,单细胞和树突细胞.
- 自然杀手T (NKT) 细胞对于弥合先天性和适应性免疫是至关重要的,并与自身免疫性疾病有关.
- 以前的研究表明,NKT细胞在自身免疫性甲状腺疾病 (AITD) 病原发生过程中可能发挥作用,可能是通过TSHR相互作用.
研究的目的:
- 为了研究NKT细胞上TSHR表达的存在或不存在.
- 为了确定TSHR在NKT细胞上的表达是否在患有和没有自身免疫性甲状腺疾病 (AITD) 的患者之间有所不同.
- 澄清 NKT 细胞在 AITD 免疫病原发生过程中的潜在直接参与.
主要方法:
- 周围血液单核细胞 (PBMC) 和孤立的NKT细胞来自86名患有良性结节性甲状腺疾病 (有或没有AITD) 和健康对照患者.
- 使用光激活细胞分类 (FACS) 来分析NKT细胞上的TSHR表达.
- 反转录聚合酶链反应 (RT-PCR) 用于确认健康个体的TSHR表达发现.
主要成果:
- 在AITD或非AITD患者的NKT细胞上没有检测到TSHR,也没有在健康个体中检测到TSHR.
- 发现TSHR在总PBMC群体 (2.77%的TSHR+细胞) 上表达.
- 在健康对照组中,RT-PCR证实了NKT细胞上没有TSHR (p < 0.0001).
结论:
- NKT细胞不会表达TSHR,这与其存在于其他免疫细胞的潜在影响相反.
- 这项研究提供了证据,证明NKT细胞通过TSHR直接影响AITD的发展.
- 这些发现需要重新评估将NKT细胞直接与自身免疫性甲状腺疾病的病变发生联系的拟议机制.
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