相关实验视频

Updated: Jun 7, 2025

An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells

An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells

Published on: September 12, 2019

滚动翻译 circRUNX2.2 在马雷克的疾病模型中促进淋巴瘤细胞增殖和循环过渡

Lulu Wang^1,2, Gang Zheng¹, Yuqin Yang¹

¹Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.

International journal of molecular sciences

|November 9, 2024

概括

马雷克病 (MD) 研究揭示 circRUNX2.2,一个圆形RNA,转化为促进淋巴瘤细胞增殖和抑制细胞亡的. 这一发现为MDV诱导的淋巴瘤病原体提供了新的见解.

科学领域:

在瘤学瘤学.
病毒学病毒学
分子生物学分子生物学

背景情况:

马雷克病 (MD) 是一种由马雷克病病毒 (MDV) 引起的免疫抑制性疾病,是淋巴瘤研究的典范.
循环RNAs (circRNAs) 越来越多地被认为是它们在各种生物过程中的作用,包括癌症.

研究的目的:

为了研究circRUNX2.2在MDV感染的瘤脏中升级的作用,在淋巴瘤细胞中.
阐明circRUNX2.2及其编码的翻译机制和生物功能.

主要方法:

在circRUNX2.2.2.中的开放阅读框架 (ORF) 的生物信息预测.
西方涂抹和多体组分测试检测和确认circRUNX2.2-衍生的翻译 (circRUNX2.2-rt).
细胞培养实验 (MSB1细胞),甲酸盐 (NaB) 处理,电子显微镜,免疫沉和质谱.

主要成果:

在MDV感染的瘤脏中,circirRUNX2.2的调节显著上升.
证实circRUNX2.2通过滚动圆机制转化为 (circRUNX2.2-rt).
circRUNX2.2-rt促进了MSB1细胞增殖,细胞周期进展 (S到G2阶段),并抑制了细胞亡.
circRUNX2.2-rt与参与细胞循环调节的蛋白质相互作用.

结论:

circRUNX2.2是一个可翻译的circRNA,产生功能性 (circRUNX2.2-rt).
circRUNX2.2-rt在促进MDV诱导的淋巴瘤细胞增殖和存活方面发挥着重要作用.
circRUNX2.2-rt代表了马雷克病的潜在治疗标.

关键词:

在CircRUNX2.2-rt中使用.马雷克的疾病是马雷克的疾病.这些都是肉肉.淋巴瘤淋巴瘤是什么滚动圆形的方式

更多相关视频

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

Published on: November 10, 2017

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Inhibition of Cdk Activity

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

The Cell Cycle Control System

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...

Positive Regulator Molecules

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.