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类似妊娠期糖尿病的燃料损害了线粒体功能和长链脂肪酸吸收在人类热囊细胞中

Kyle M Siemers¹, Lisa A Joss-Moore², Michelle L Baack^3,4

¹Sanford School of Medicine, University of South Dakota, 414 E. Clark Street, Vermillion, SD 57069, USA.

International journal of molecular sciences

|November 9, 2024

概括

孕期糖尿病 (GDM) 损害了胎盘脂肪酸 (FA) 的吸收和脂质滴存储,特别是高葡萄糖和脂质的联合. 这有助于胎儿长链多不和脂肪酸 (LCPUFA) 缺乏和不良结果.

科学领域:

生殖生物学生殖生物学
孕产妇和胎儿医学孕产妇和胎儿医学
细胞的新陈代谢

背景情况:

孕期糖尿病 (GDM) 会导致母亲的高血糖和高脂血症.
暴露于GDM的婴儿面临长链多不和脂肪酸 (LCPUFA) 缺乏症的风险.
连接糖尿病燃料与胎盘脂肪酸 (FA) 运输和脂质滴滴动态的机制尚不清楚.

研究的目的:

为了研究高葡萄糖和高脂条件,单独和组合,如何影响 trofhoblast 功能.
分析对热原体生长,活力,线粒体功能,FA吸收和脂质滴滴分区的影响.
阐明GDM相关的代谢变化如何破坏胎盘脂质处理.

主要方法:

使用的BeWo和初级分离细胞质细胞 (CTBs) 暴露于不同的葡萄糖和脂质度.
评估了热囊细胞的生长,生存能力和亡.
测量了线粒体的生物能量和BODIPY标记的脂肪酸 (FA) 摄入量,包括特定的链条长度 (C12,C16).
分析了脂质滴滴数,面积和亚细胞局部.

主要成果:

吸收FA对细胞中的酸链长度和媒体暴露敏感.
结合高葡萄糖和高脂暴露增加了线粒体蛋白质,但导致呼吸能力受损和由于亡而降低活力.
暴露在组合条件下的热囊细胞显示,BODIPY C16在细胞和脂质滴中吸收率降低,脂质滴特征发生变化,与单个暴露不同.

结论:

结合高葡萄糖和脂质条件在GDM显著扰乱胎盘FA运输和脂质滴滴动态.
这些干扰会损害 trofhoblast 功能和活力,这可能解释新生儿的 LCPUFA 缺乏.
这些发现突显了GDM对胎盘脂质代谢的复杂影响,有助于妊娠不良后果.

关键词:

脂肪酸脂肪酸脂肪酸脂肪酸孕期糖尿病孕期糖尿病脂质滴滴滴滴滴滴滴滴滴滴滴滴滴线粒体中的线粒体.热囊细胞的热囊细胞.

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