在寄生性平坦中鉴定出具有曲螺旋基因的抗菌,Mesocestoides corti
Tomislav Rončević1, Marco Gerdol2, Sabrina Pacor2
1Department of Biology, Faculty of Science, University of Split, 21000 Split, Croatia.
研究人员发现了mesco-2,这是一种来自寄生虫Mesocestoides corti.的新型抗微生物 (AMP). 这种强效的具有广泛的抗菌活性和独特的膜向机制,提供了一个有前途的新型抗生素替代品.
科学领域:
- 寄生虫学的寄生虫学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 抗生素耐药性的增加需要发现新型抗菌剂.
- 抗微生物 (AMP) 是一类有前途的治疗药物,但寄生虫衍生的AMP在很大程度上尚未被探索.
- Mesocestoides corti是一种寄生性平虫,有可能发现新的生物活性化合物.
研究的目的:
- 识别和描述来自寄生虫平虫Mesocestoides corti.的新型抗菌.
- 评估最有前途的候选者mesco-2的抗菌活性和作用机制.
- 为了阐明mesco-2抗微生物功能的结构基础.
主要方法:
- 全基因组挖掘用于识别Mesocestoides corti.中的潜在AMP前体.
- 化学合成和mesco-2的综合性表征.
- 在体外抗菌试验对一组格兰氏阴性和格兰氏阳性细菌进行检测.
- 循环二重化谱法用于研究不同环境中的二次结构.
- 结构预测和分子动力学模拟以建模膜相互作用.
主要成果:
- 确定了三种新的AMP前体 (mesco-1, -2, -3),其中mesco-2显示出显著的潜力.
- 合成的mesco-2表现出强大的广泛抗菌活性,可以对抗大肠杆菌,肺炎,宝曼尼,P. aeruginosa和金黄色.
- 机理学研究揭示了一种膜破坏机制,mesco-2选择性地准离子细菌膜.
- 循环二元化和模拟表明mesco-2在阳离子膜上采用曲螺旋形状,由特定的氨基酸动机驱动.
结论:
- 梅斯科-2是一种强效的,广泛的抗微生物,来自寄生虫平虫.
- 它的选择性膜相互作用和独特的结构特征使其成为新型抗生素开发的有希望的候选者.
- 这项研究强调了寄生虫衍生分子作为创新抗菌剂来源的潜力.
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