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Updated: Jun 7, 2025

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Published on: October 8, 2018

在tRNA基因中的DNA的结构特征及其上游序列

Ekaterina A Savina^1,2, Tatiana G Shumilina², Viktoria A Porolo²

¹Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.

International journal of molecular sciences

|November 9, 2024

概括

这项研究确定了在真核生物,古生物和细菌中保存的tRNA基因促进元件 (盒子A和盒子B). 然而,侧边区域的DNA结构性质有很大差异,TATA结合蛋白位点只保留在特定物种中.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
生物信息学是一种生物信息学.

背景情况:

RNA聚合酶III (Pol III) 使用具有TFIIIC认可的内部控制区域 (盒子A,盒子B) 的II型促进体转录tRNA基因.
tRNA基因的5'-侧翼区域对于转录调节至关重要,尽管共识序列并不普遍保存.
塔塔结合蛋白 (TBP) 是一种普遍的真核转录因子,在古生物中存在一个正方体.

研究的目的:

在真核生物和古生物中检测内部和外部的Pol III促进子位置,调节tRNA基因转录.
分析具有和没有TBP的生物体中tRNA基因侧边区域的DNA特性.

主要方法:

在tRNA基因区域中分析文本,结构,机械和物理化学DNA特性.
使用来自GtRNAdb数据库的11种生物的代表性tRNA基因组.
对细菌,古生物和真核生物进行比较分析.

主要成果:

在所有三个域 (真核生物,古生物,细菌) 的tRNA基因中发现了相同的共识序列和A和B盒的位置.
在域之间和域内观察到5'-边缘区域的DNA结构和机械性质的显著变化.
保存的TATA结合蛋白 (TBP) 结合位点仅在*S. pombe*和*A. thaliana*中被确定.

结论:

虽然核心tRNA促销元件被保留,但调节性5'-侧翼区域表现出相当大的进化分歧.
侧边区域的变异性表明tRNA基因转录在不同生命领域的多种调节机制.
TBP绑定站点的有限保存表明了特定领域的角色或替代的招聘策略.

关键词:

DNA局部空间结构的局部空间结构 DNA与蛋白质的相互作用 DNase I 分裂的时间的RNA聚合酶III.在TBP中,TBP是TBP.考古学是指古物质 (archaea) 是指古物质.细菌细菌细菌是一种细菌.它们是真核生物 (eukaryotes).监管地区的监管地区.在tRNA转录过程中,超声波切割超声波切割

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