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来自Dendrobium officinale的甘油脂的制备过程优化以及与 Askorbic 酸相比抗氧化作用的差异

Yan Long¹, Jiajing Yang¹, Hongfei Ji¹

¹Yantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, School of Life Sciences, Yantai University, Yantai 264005, China.

|November 9, 2024

概括

丹德罗 (Dendrobium officinale) 的糖脂 (DOG) 被有效地提取出来,并被确定为二甲糖化物. 通过调节肠道微生物群,DOG表现出强大的抗氧化作用,为功能性食品开发提供了潜力.

科学领域:

* 植物化学植物化学
* 营养保健品营养保健品
* 微生物学微生物学

背景情况:

* Dendrobium officinale glycolipids (DOG) 是多糖提取的副产品. 这些是多糖提取的副产品.
*目前的方法往往会丢弃这些有价值的葡萄糖脂类残留物.

研究的目的:

*为了优化从D. officinale残留物中提取DOG.
* 描述DOG的结构和抗氧化特性.
* 评估通过肠道微生物群代谢介导的DOG的抗氧化作用.

主要方法:

*采用响应表面方法来确定最佳的提取参数 (液体-固体比,乙醇度,温度,时间).
*分析了DOG的结构性质.
*DOG的抗氧化作用与活体中亚酸相比较,重点关注肠道微生物群调节和相关生物标志物.

主要成果:

* 在特定条件下 (20毫升/克,70%乙醇,70°C,2.5小时) 达到2.64%的最佳DOG提取产量.
* DOG被确定为富含葡萄糖,曼诺斯,酸和9,12,15-octadecatrienoic酸的二甲酸,具有直接的激素清除活性.
* 在小鼠中,DOG增强了肠道的Bacteroides acidifaciens,调节了特定的基因表达,并改善了抗氧化酶活动 (SOD,GSH-Px),同时减少了氧化应激标志物 (MDA).

结论:

* Dendrobium officinale glycolipids (DOG) 是有效提取的具有显著抗氧化潜力的二甲酸糖.
* DOG主要通过调节肠道菌群代谢来产生抗氧化作用.
* 调查结果支持对D. officinale残留物的价值化和DOG作为功能性食品成分的发展.

关键词:

丹德罗比亚姆官员的糖脂类.抗氧化作用具有抗氧化作用.肠道菌群的代谢,肠道菌群的代谢.结构分析是结构分析.

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