赛卡斯介质的抗癌作用:通过调节PI3K/AKT/mTOR信号通路的分子基础
Jawaher Alqahtani1, Esraa M Mosalam2,3, Hend E Abo Mansour2,4
1Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11495, Saudi Arabia.
这项研究表明,Cycas媒体提取物具有显著的抗瘤和抗菌特性. 它有效地减少了癌细胞的增殖,并在体内抑制了瘤的生长,同时也对抗金黄色葡萄球菌感染.
科学领域:
- 植物化学 植物化学
- 药理学 药理学是指药理学的学科.
- 微生物学 微生物学
背景情况:
- 由于植物的安全性和潜在的药理学益处,植物被广泛选为生物活动.
- Cycas media 是一种具有潜在药用应用的植物物种,需要进行研究.
研究的目的:
- 评估Cycas媒体甲醇提取物对HepG2肝癌细胞的抗增殖和细胞毒性作用.
- 评估提取物对固体埃里希癌模型的抗瘤活性,并阐明其分子机制.
- 为了研究Cycas媒体提取物对黄金葡萄球菌的抗菌特性.
主要方法:
- 细胞毒性和细胞周期分析是在用不同度的提取物治疗的HepG2细胞上进行的.
- 在体内研究中,向瘤携带的小鼠注射100和200毫克/千克的C.媒体提取物,监测瘤体积,抑制率,毒性和抗氧化生物标志物.
- 我们分析了PI3K/AKT/mTOR信号通路,以了解抗瘤机制. 通过最小抑制度 (MIC) 和膜完整性测试来评估抗菌活性.
主要成果:
- 西卡斯介质提取物对HepG2细胞表现出显著的抗增殖作用,并抑制PI3K/AKT/mTOR通路.
- 在体内,提取物显著抑制瘤生长,逆转了抗氧化生物标志物 (SOD,GPX4,GSH,MDA) 的负面变化,高剂量显示出更高的疗效.
- 提取物表现出对黄金葡萄球菌的抗菌活性,MICs范围为32至128μg/mL,以及细菌膜完整性受损.
结论:
- 锡卡斯介质提取物具有有前途的抗瘤潜力,部分通过抑制PI3K/AKT/mTOR通路和抗氧化作用进行调解.
- 提取物还显示出明显的抗菌活性对金黄色葡萄球菌,表明其在打击细菌感染的实用性.
- 这些发现凸显了Cycas媒体作为开发癌症和细菌疾病的新疗剂的潜在来源.
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