开发一个分离系统,以便在发酵过程中实时回收乙-布坦醇
Christopher Chukwudi Okonkwo1,2,3, Ademola Duduyemi1, Victor Chinomso Ujor4
1Department of Animal Science, College of Food Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH, 44691, USA.
Applied microbiology and biotechnology
|November 9, 2024
概括
这项研究引入了一种疏水网,可以改善生物反应器在乙-布坦醇-乙醇 (ABE) 发酵过程中保持水分. 开发出来的网格增强了回收流中的butanol度,有助于有效的ABE回收.
科学领域:
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
- 材料科学 材料科学 材料科学
背景情况:
- 乙-布坦醇-乙醇 (ABE) 发酵的真空辅助气体剥离 (VAGS) 会导致生物反应器的大量水损失,需要补充水.
- 有效回收ABE产品对于优化发酵过程和经济可行性至关重要.
研究的目的:
- 开发和评估一种疏水性不钢网格,用于在VAGS过程中选择性回收缩的ABE流.
- 评估网格在生物反应器内保留水的能力,并提高产品度.
主要方法:
- 使用氧化 (ZnO) 和聚二甲基 (PDMS) 制造疏水性和油性不钢网格 (180微米,300微米,425微米).
- 将网格集成到5L生物反应器中,以评估从具有不同丁醇度 (3-10 g/L) 的模型溶液中选择性ABE提取.
- 使用无细胞和富含Clostridium beijerinckii的ABE模型溶液进行测试,以评估在发酵条件下的性能.
主要成果:
- 与未涂层网格相比,180μm的ZnO/PDMS涂层网格显著改善了水的保留,在没有细胞的情况下保留了54-64%的水,在细胞的情况下保留了61-65%的水.
- 使用180微米ZnO-PDMS网格回收的冷凝液中的butanol度高达未涂层网格的10.8倍.
- 疏水性网状物证明了与纤维素酸发酵的兼容性,并防止了分离系统的堵塞.
结论:
- 开发的ZnO-PDMS疏水网有效地回收缩的ABE,同时在生物反应器中选择性地保留水.
- 这项技术提供了一个有前途的解决方案,用于在不堵塞系统的情况下实时回收在线纤维素和体材料发酵中的ABE.
- 疏水网所获得的增强的保留水和产品度有助于更高效和可持续的生物处理.
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