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Updated: Jun 12, 2025

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来自人类B细胞的Pseudomonas aeruginosa III型分泌系统中和单克隆抗体的开发协议

Alexandra Albus1, Christoph Kreer2, Florian Klein3

  • 1Department I of Internal Medicine, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50937 Cologne, Germany; Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.

STAR protocols
|November 9, 2024
PubMed
概括

开发人类单克隆抗体来对抗Pseudomonas aeruginosa.

关键词:
生物技术和生物工程 生物技术和生物工程细胞生物学 细胞生物学细胞培养培养的细胞培养.细胞隔离细胞隔离基于细胞的测定.免疫学 免疫学 免疫学微生物学的微生物.分子生物学分子生物学一个单细胞的单细胞.

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
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