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拼接因子SF3B1对于早期猪胚胎的适当替代拼接和胚胎基因组激活至关重要
Yanan Zhao1, Hua Zhang2, Benliang Zhou1
1College of Animal Science & Technology, Guangxi University, Nanning, Guangxi 530004, China; Guangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, Nanning, Guangxi 530004, China.
International journal of biological macromolecules
|November 9, 2024
概括
替代拼接对于胚胎发育至关重要. 干扰猪胚胎中的剪接因子SF3B1会在4细胞阶段停止发育,影响胚胎基因组激活和新陈代谢.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 替代拼接 (AS) 是胚胎发育中的一个关键的转录后调节.
- 在早期的猪胚胎中,在囊胚基因组激活 (ZGA) 过程中AS的特定作用尚不清楚.
研究的目的:
- 调查猪胚胎中ZGA期间替代拼接事件 (ASEs) 的流行率和模式.
- 确定破坏剪接因子3B子单元1 (SF3B1) 对早期猪胚胎发育,ZGA和代谢平衡的影响.
主要方法:
- 在ZGA期间对猪胚胎中的ASE的分析.
- 在猪胚胎中使用pladienolide B (PlaB) 抑制SF3B1.
- 评估ZGA基因表达 (DPPA2,EIF6,SORD) 和拼接模式.
- 评估胚胎代谢变化,包括LDHA/LDHB比率和乳酸水平.
- 对基因素乳化修饰的分析 (pan-Kla,H4K5la).
主要成果:
- 在哺乳动物胚胎中,在ZGA期间,ASE最为普遍,跳过的外形是主要的模式.
- 通过PlaB抑制SF3B1,导致猪胚胎在4细胞阶段停止.
- SF3B1的破坏导致关键ZGA基因 (DPPA2,EIF6,SORD) 的异常拼接,表明ZGA失败.
- 胚胎代谢平衡被破坏,由增加的LDHA/LDHB比率,乳酸和基因素乳化证明.
结论:
- 替代拼接在猪肉细胞基因组激活和早期胚胎发育中起着至关重要的作用.
- 在猪胚胎中,SF3B1对于维持新陈代谢平衡和成功的ZGA至关重要.
- 了解这些机制可以帮助解决与胚胎发育相关的生殖挑战.
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