气味剂2 - 甲基利索醇的生物合成是在蛋白质外内进行细分的
Michael P Andreas1, Tobias W Giessen2
1Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI, 48109, USA.
Nature communications
|November 9, 2024
概括
蛋白Esha,现在Sg Enc,是一种细菌封装蛋白蛋白,而不是转录因子. 它形成纳米隔间封装2 - 甲基伊索博醇合成酶,影响类生物合成.
科学领域:
- 微生物学 微生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 类是各种各样的天然产品,在各种生物过程中发挥着重要作用.
- 2-甲基二氧化 (2-MIB) 是一种由细菌产生的挥发性化物,导致水中的土味气味和异味.
- 蛋白质EshaA以前被描述为一种转录因子,它参与了Streptomyces griseus的2-MIB生物合成.
研究的目的:
- 重新评估Streptomyces griseus中的Esha蛋白的功能.
- 阐明Sg Enc的结构和功能特性及其在2-MIB生产中的作用.
- 确定2B家族囊素在细菌二次代谢物生物合成中的作用.
主要方法:
- 使用冷电子显微镜 (cryo-EM) 确定了Sg Enc.的结构.
- 生物化学试验被用来研究Sg Enc域的结合特性和封装的2-MIBS的活性.
- 进行了蛋白质-蛋白质相互作用和域函数的分析.
主要成果:
- Sg Enc被认定为2B家族的封闭素外蛋白,形成一个二面体结构.
- Sg Enc封装了2-甲基利索波醇合成酶 (2-MIBS),形成了一个蛋白质纳米隔间.
- Sg Enc上的循环腺单酸盐 (cAMP) 结合域 (CBD) 不结合cAMP,cAMP也不调节2-MIBS活动或Sg Enc结构.
- 货物装载是由Sg Enc的N端无序域介导的.
结论:
- EshA的功能从转录因子重新定义为细菌纳米区的结构组成部分.
- 2B家族的囊素作为载荷蛋白质结构,参与细菌的二次代谢物生物合成.
- 这项研究为细菌封装素的结构组织和功能作用提供了新的见解.
相关概念视频
Eukaryotic Compartmentalization
10.6K
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal...
For example, lysosomes in the animal...
10.6K
Biosynthesis in Bacteria
1
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
1
Structure of Porins
2.9K
Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel...
2.9K
Biosynthesis of Lipids
1
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
1
Porin Insertion in the Outer Mitochondrial Membrane
2.8K
Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane.
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
2.8K
Protein Transport to the Inner Chloroplast Membrane
2.1K
Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
2.1K


