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建立潜在的lncRNA相关的枢纽基因涉及到在肺腺癌中具有竞争力的内源性RNA

Yong Li¹, Danfei Shi², Yan Jiang³

¹Department of Laboratory Medicine, First Affiliated Hospital of Huzhou University, Huzhou, 313000, Zhejiang, China.

|November 10, 2024

概括

长非编码RNAs (lncRNAs) 可以帮助诊断和预测肺腺癌 (LUAD) 的结果. 使用 lncRNA 相关的枢纽基因 (LRHGs),特别是 HOXD-AS2 的模型,显示了 LUAD 诊断和预后的前景.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

长非编码RNAs (lncRNAs) 在癌症诊断和预后中发挥作用.
与lncRNA相关的枢纽基因 (LRHGs) 和肺腺癌 (LUAD) 结果之间的特定关联需要进一步阐明.

研究的目的:

建立基于LRHGs的LUAD诊断和预后模型.
在LUAD中调查血清HOXD集群抗理性RNA 2 (HOXD-AS2) 的诊断价值.
探索LUAD中LRHG的潜在机制和相关性.

主要方法:

利用癌症基因组图谱 (TCGA) -LUAD数据集的最小绝对收缩和选择操作员 (LASSO) 分析来构建一个LRHG模型.
采用基因组丰富分析 (GSEA) 和基因组变异分析 (GSVA) 来研究LRHG机制.
评估了血清HOXD-AS2的诊断作用,使用接收器操作特征 (ROC) 曲线分析和对相关分子的皮尔森相关性分析.

主要成果:

确定了141个差异表达的lncRNA,并构建了9个LRHG的预后特征,用于LUAD的整体存活率.
与对照组相比,HOXD-AS2表达在LUAD组织和血清中显著升高,显示出比CYFRA21-1更高的诊断准确性.
在LUAD中,HOXD-AS2表达与miR-4538负相关,与calmodulin依赖蛋白激酶II型β子单元 (CAMK2B) 有正相关.

结论:

开发的LRHG模型,以HOXD-AS2作为关键标志物,可以独立诊断和预测LUAD预后.
建议HOXD-AS2/miR-4538/CAMK2B轴作为LUAD的一个潜在机制.
这些发现可能为LUAD治疗提供新的策略.

关键词:

诊断诊断诊断诊断诊断这是一个LncRNARNA.肺部腺癌瘤是肺部腺癌.ceRNARNA是什么意思

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