相关实验视频

Updated: Jun 7, 2025

Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry

Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry

Published on: May 24, 2024

线粒体动力学和能量代谢干扰疗法促进光热敏感化

Cuimei Liu¹, Sihang Cheng¹, Xue Zhou²

¹College of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun 130024, PR China; College of Chemical and Materials Engineering, Bohai University, 19 Science and Technology Road, Jinzhou 121013, PR China.

Journal of colloid and interface science

|November 10, 2024

概括

这项研究引入了针对癌细胞线粒体和细胞质的多功能纳米薄膜. 这些纳米薄膜通过破坏能量产生和抑制热冲击蛋白 (HSP) 来提高光热疗法 (PTT) 的有效性,克服瘤的耐热性.

科学领域:

生物医学工程生物医学工程
材料科学材料科学材料科学
癌症治疗癌症治疗

背景情况:

光热疗法 (PTT) 是有效的,但受到热冲击蛋白 (HSP) 过度表达的限制,导致细胞热阻塞.
线粒体在HSP生产和能源供应中发挥着关键作用,但线粒体动力学可以赋予对PTT的抵抗力.
破坏线粒体动力学对于抑制HSP产生和增强PTT疗效至关重要.

研究的目的:

通过准线粒体和糖解,开发用于增强PTT的多功能纳米片.
调查线粒体动力学和能量生产的破坏,以改善癌症治疗.
通过协同治疗策略克服PTT中的瘤耐热性.

主要方法:

合成可降解的Cu3BiS3 (CBS) 纳米薄膜,改用三 (TPP) 进行线粒体向和β-环氧 (CD) 进行二氧化葡萄糖 (2DG) 加载.
研究TPP修饰在增加活性氧物种 (ROS) 和动氨酸相关蛋白1 (Drp1) 表达中的作用,破坏线粒体平衡.
利用2DG抑制糖解和减少ATP的产生,导致细胞饥饿和减少HSP的合成.

主要成果:

TPP-CBS-2DG Janus纳米片 (JNSs) 有效地准了线粒体,并破坏了线粒体的动态平衡.
JNSs抑制了线粒体和糖质 ATP 生产,诱导细胞饥饿.
该JNS显著提高了瘤的热敏感性,通过抑制HSP的产生来提高PTT的疗效.

结论:

TPP-CBS-2DG JNSs提供了一种有希望的策略,以克服PTT中的瘤耐热性.
开发的多功能纳米片为选择性修改的多功能纳米材料制造提供了可行的方法.
这种方法有可能通过将PTT与代谢向相结合来推进癌症治疗.

关键词:

降解降解是一种降解.能量代谢能量代谢雅努斯纳米薄膜是如何使用的线粒体动力学的动态敏感的光热治疗疗法敏感的光热治疗针对线粒体的目标

更多相关视频

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Published on: February 3, 2023

Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

Near Infrared NIr Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium

Published on: March 14, 2015

相关概念视频

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

Mitochondrial Membranes

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...