通过酶固定化扩大糖糖合成酶反应的高pH范围
Hui Liu1, Annika J E Borg2, Bernd Nidetzky2
1Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, NAWI Graz, Graz, Austria.
Journal of biotechnology
|November 10, 2024
概括
在纳米纤维素载体上固定糖合成酶 (SuSy),在高pH下增强其活性,从而使用尿素5'-二酸盐 (UDP) - 葡萄糖实现高效的酶性糖化. 这克服了可溶性SuSy的局限性,在性条件下实现高糖产量.
科学领域:
- 生物化学 生物化学
- 酶工程是什么?酶工程是什么?
- 生物技术是生物技术.
背景情况:
- 由于质子释放,高pH值在热力学上有利于糖化反应.
- 糖糖合成酶 (SuSy) 对于糖糖合成至关重要,但在性pH下会失去活性.
- 在高pH下优化SuSy活性可以提高酶性糖化效率.
研究的目的:
- 扩大糖合成酶 (SuSy) 的性pH范围,用于酶性糖化.
- 为了克服通过固定化在高pH下SusY生产力的损失.
- 在性条件下使用固定式SuSy来证明高效的糖糖合成.
主要方法:
- 在纳米纤维素基生物复合材料载体上固定大豆SusY.
- 固定式SuSy.的pH-生产力概况的表征.
- 使用固定式SuSy和自动化pH控制的糖糖的酶合成.
主要成果:
- 固定后的SuSy在pH值9.5下保持了高达80%的最大容量,显著扩大了其操作pH值范围.
- 通过在pH值~9.0.0下使用固定的SuSy,实现了UDP-葡萄糖和果糖几乎完全转化 (≥96%) 为糖糖.
- 固定化在性条件下显著改善了SuSy的稳定性和活性.
结论:
- 使用糖核酸捐赠体的酶基糖化可以通过高pH有效地驱动.
- 固定是SuSy运营活动适应高pH环境的关键策略.
- 这种方法促进了高效和热力学上有利的糖糖合成.
相关概念视频
02:48Henderson-Hasselbalch Equation
The ionization-constant expression for a solution of a weak acid can be written as:
03:55Titration Calculations: Weak Acid - Strong Base
Calculating pH for Titration Solutions: Weak Acid/Strong Base
For the titration of 25.00 mL of 0.100 M CH3CO2H with 0.100 M NaOH, the reaction can be represented as:
For the titration of 25.00 mL of 0.100 M CH3CO2H with 0.100 M NaOH, the reaction can be represented as:
02:11Leveling Effect and Non-Aqueous Acid-Base Solutions
This lesson defines the leveling effect in acidic and basic solutions and its role in aqueous and non-aqueous solutions. It is essential to understand the competing nature of various species in a chemical system.
The Leveling Effect of a Solvent
A generic acid (HA) reacts with the generic base (B-) to yield the corresponding conjugate base (A-) and conjugate acid (HB):
The Leveling Effect of a Solvent
A generic acid (HA) reacts with the generic base (B-) to yield the corresponding conjugate base (A-) and conjugate acid (HB):
01:20Titration of a Weak Base with a Strong Acid
The titration curve of a weak base like ammonia with a strong acid like hydrochloric acid is the mirror image of the titration curve of a weak acid with a strong base.
Using the ICE table and substituting the Kb value, we calculate the initial pH of 50 mL of 0.1 M ammonia to be 11.11. Addition of 25 mL of 0.1 M hydrochloric acid to this solution of ammonia results in a buffer with an equal concentration of ammonia and ammonium ions. The pH of this buffer can be calculated by substituting these...
Using the ICE table and substituting the Kb value, we calculate the initial pH of 50 mL of 0.1 M ammonia to be 11.11. Addition of 25 mL of 0.1 M hydrochloric acid to this solution of ammonia results in a buffer with an equal concentration of ammonia and ammonium ions. The pH of this buffer can be calculated by substituting these...
00:53Extraction: Effects of pH
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01:23Titration of a Strong Acid with a Strong Base
During the titration of a strong acid with a strong base, pH calculations are primarily based on the concentration of residual hydronium or hydroxide ions. Initially, a strong acid like hydrochloric acid fully dissociates, creating hydronium and chloride ions, resulting in a low pH. The addition of a strong base like sodium hydroxide alters the concentration of hydronium ions by neutralizing them. As more base is added, the pH gradually increases. At the equivalence point, all hydronium ions...