无抗生素高水平的l-甲氨酸在工程大肠杆菌中产生
Lijuan Wang1,2, Yingying Guo1,2, Mengyue Li1,2
1The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, P. R. China.
Journal of agricultural and food chemistry
|November 11, 2024
概括
研究人员增强了微生物生产的l-氨酸,一个关键的氨基酸. 他们使用基因工程和无抗生素系统实现了更高的产量,为成本有效的制造铺平了道路.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 工业微生物学 工业微生物学
背景情况:
- 甲氨酸是一种重要的含硫氨基酸,具有多种工业应用.
- 微生物l-氨酸生产的挑战包括复杂的生物合成和等离子体不稳定性.
- 需要有效且具有成本效益的生产方法.
研究的目的:
- 为了改善微生物生物合成的l-氨酸.
- 开发一种无抗生素的发酵系统,以提高产量.
- 为了研究克服发酵过程中等离子体损失的策略.
主要方法:
- 代谢工程策略:增强糖-CoA供应,引入直接硫化,削弱l-threonine通路.
- 纳入Hok/Sok系统以稳定等离子体并使无抗生素的种植成为可能.
- 在5升生物反应器中进行料批发发酵,以评估生产效率.
主要成果:
- 工程菌株实现了21.55g/L的l-氨酸,其产量为0.14g/g的葡萄糖.
- 没有抗生素的系统 (等离子体pAm10) 使得M2菌株 thrBA1G能够产生20.39g/L l-methionine.
- 与没有抗生素的对照组相比,观察到l-氨酸生产增加了42.58%.
结论:
- 代谢工程和霍克/索克系统显著增强了l-氨酸的生产.
- 无抗生素,基于等离子体的发酵是一种可行的策略,用于高效和成本效益的l-氨酸制造.
- 这种方法对其他有价值的化学品和氨基酸的可持续生产有潜力.
相关概念视频
01:23Stringent Response in E. coli
533
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
533
01:27Production of Antibiotics
281
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
281
01:30Production of Pharmaceuticals
99
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
99