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相关概念视频

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

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相关实验视频

Updated: Jul 7, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

31.4K

smFRET辅助的RNA结构预测

Jun Li1, Nils G Walter2, Shi-Jie Chen3

  • 1Department of Physics, University of Missouri, Columbia, MO, USA.

Communications in information and systems
|November 11, 2024
PubMed
概括

我们开发了一种新的方法,将单分子福斯特共振能量转移 (smFRET) 与分子动力学模拟相结合,以预测RNA结构. 这种方法在拼接过程中准确地模拟了mRNA前的形状变化.

关键词:
预测RNA结构 预测RNA结构在Ubc4前mRNA中.粗粒度的MD模拟模型这是一个smFRETET.

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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相关实验视频

Last Updated: Jul 7, 2026

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Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
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科学领域:

  • 生物物理学的生物物理.
  • 计算生物学 计算生物学
  • 分子生物学分子生物学

背景情况:

  • 单分子福斯特共振能量转移 (smFRET) 为分子动力学提供了洞察力.
  • 之前的研究使用smFRET用于RNA拼接动态,但缺乏详细的结构建模.
  • 了解RNA结构对于破译生物功能至关重要.

研究的目的:

  • 开发和验证smFRET辅助的计算方法,用于预测RNA的二维和三维结构.
  • 在拼接过程中模拟截断的Ubc4前mRNA的构造动态.
  • 将实验smFRET数据与分子动力学模拟集成,以提高结构预测.

主要方法:

  • 使用粗粒度分子动力学 (MD) 模拟生成RNA结构合集.
  • 通过染料组的全原子MD模拟计算染料间距离.
  • 将模拟距离与实验smFRET数据进行比较,以确定RNA结构.

主要成果:

  • 开发的方法成功预测了截断的Ubc4前mRNA的2D和3D结构.
  • 计算预测显示了与实验smFRET发现的显著对齐.
  • 在拼接过程中,在二维层面发现了一个关键的形状变化.

结论:

  • smFRET辅助的预测方法对于RNA结构建模是有效的.
  • 这种方法增强了对RNA结构动态的理解.
  • 这些发现为未来研究RNA结构功能关系的研究提供了基础.