多种组分的分析揭示了在HTR-8/SVneo细胞中氨酸诱导的生殖毒性
Qibin Wu1, Mengting Chen2, Yifan Lin2
1Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou 350001, China.
Heliyon
|November 11, 2024
概括
库尔迪昂是Curcumae Radix中的一种化合物,可能通过破坏细胞过程和破坏DNA来损害生殖细胞. 这项研究调查了其在胎盘细胞中的毒性机制,揭示了怀孕期间的潜在风险.
科学领域:
- 药理学 药理学是指药理学的学科.
- 生殖毒理学 生殖毒理学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 怀孕期间的药物安全性至关重要.
- 来自Curcumae Radix的curdione已经显示出治疗潜力,但其生殖毒性是未知的.
- 了解氨酸的作用机制对于评估其安全性至关重要.
研究的目的:
- 为了研究 HTR-8/SVneo 胎盘细胞中氨酸诱导的毒性机制.
- 探索表观遗传学,蛋白质学和代谢学在子毒性的作用.
- 验证与氨酸对生殖细胞的影响相关的实验发现.
主要方法:
- 利用表观遗传学,蛋白质学和代谢学来分析库尔迪昂的作用.
- 评估了m6A修饰,基因表达和蛋白质水平的改变.
- 进行实验验证观察到的细胞损伤和通路参与.
主要成果:
- 库尔迪改变了HTR-8/SVneo细胞中的m6A修饰,基因表达和细胞代谢.
- 库尔迪昂诱导了氧化应激,线粒体损伤和DNA损伤.
- 观察到Wnt6,β-catenin,ZO-1和CLDN1蛋白的下调,表明Wnt/β-catenin通路的破坏.
结论:
- 库尔迪通过氧化应激和线粒体功能障碍诱导HTR-8/SVneo细胞损伤.
- Wnt/β-catenin信号通路与皮素诱导的紧结中断有关.
- 库尔迪昂的生殖毒性机制涉及表观遗传,蛋白质和代谢变化.
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