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通过液压冲击机的旋转发生器在水中的MS-2病毒的无活化
Benjamin Bizjan1,2, Gašper Rak2, Sabina Kolbl Repinc2,3
1University of Ljubljana, Faculty of Mechanical Engineering, Aškerčeva 6, 1000, Ljubljana, Slovenia.
Heliyon
|November 11, 2024
概括
液压冲击波通过破坏病毒囊和识别受体,有效地使MS-2细菌,一种诺罗病毒代用物失活. 这种新的方法显示出对用可扩展设备处理病毒污染水的前景.
科学领域:
- 环境微生物学环境微生物学
- 水处理技术水处理技术.
- 病毒失活机制是病毒的失活机制.
背景情况:
- 诺罗病毒污染对公众健康构成重大风险.
- 有效的病毒代用物如MS-2菌的无活化对水安全至关重要.
- 现有的水处理方法可能在效率或可扩展性方面存在局限性.
研究的目的:
- 为了研究液压冲击波对失活MS-2菌的有效性.
- 通过液压冲击波阐明病毒失活的机制.
- 评估旋转式液压冲击发生器在水处理方面的潜力.
主要方法:
- 使用旋转发电机在含有MS-2菌的水中产生液压冲击波.
- 暴露的病毒样本在受控的刀片冲击速度下重复通过液体.
- 量化病毒斑块的减少和体蛋白质的降解.
- 监测水温和空洞化水平.
主要成果:
- 在80-100次液体通过范围内,MS-2斑块数量减少了3个日志.
- 在前20次通过后,MS-2囊蛋白显著降解.
- 观察到进一步的病毒失活,归因于病毒识别受体的损伤.
- 在没有空洞化的情况下,在中等冲击波幅 (数十个MPa) 时确认了无活化功效.
结论:
- 适度振幅的液压冲击波足以进行有效的病毒失活.
- 旋转式液压冲击发生器为病毒污染水处理提供了可扩展和有前途的技术.
- 该研究强调了一种新的水消毒方法,具有潜在的公共卫生益处.
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