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在口腔状细胞癌中研究lncRNAPRKCA-AS1的表达

Dongyong Wang¹, Rui Li^1,2, Qian Liu¹

¹Oral Disease Research Center, School of Stomatology, Binjiang Campus, Wannan Medical College, Wuhu, China.

Translational cancer research

|November 11, 2024

概括

长非编码RNAPRKCA-AS1 (PRKCA-AS1) 在口腔状细胞癌 (OSCC) 中高度表达,与晚期瘤相对应. PRKCA-AS1调节OSCC细胞的增殖,迁移和入侵.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一种遗传学.

背景情况:

口腔状细胞癌 (OSCC) 是一种高度恶性口腔和面瘤,预后不佳.
长非编码RNAs (lncRNAs) 通过调节细胞行为,与癌症的发展有关.
在OSCC中lncRNA PRKCA-AS1的作用仍然在很大程度上未被探索.

研究的目的:

在OSCC中调查lncRNA PRKCA-AS1的表达水平.
探索lncRNA PRKCA-AS1在OSCC进展中的潜在机制和生物功能.

主要方法:

实时光定量聚合酶链反应 (RT-qPCR) 用于测量OSCC组织和细胞系中的PRKCA-AS1表达.
在OSCC细胞系上进行了包括细胞增殖,迁移和入侵试验在内的功能性试验.
使用PRKCA-AS1的抑制和过度表达来评估其功能影响.

主要成果:

与相邻的非癌性组织相比,OSCC组织中的PRKCA-AS1表达显著增加.
较高的PRKCA-AS1水平与瘤透深度增加,淋巴结转移和高级TNM分期相关.
抑制PRKCA-AS1抑制了OSCC细胞的增殖,迁移和入侵,而过度表达并没有在统计学上显著地改变这些过程.

结论:

LncRNA PRKCA-AS1在OSCC上调,其表达与瘤进展和转移相关.
PRKCA-AS1在调节OSCC细胞的增殖,迁移和入侵能力方面发挥着重要作用.

关键词:

长非编码RNA (lncRNA) 是一种长非编码RNA.这就是PRKCA-AS1的原因.诊断诊断诊断诊断诊断诊断口腔状细胞癌 (OSCC) 是一种治疗治疗治疗治疗治疗治疗

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