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一种基于磁分离向上转换光探针的克伦布托罗尔检测方法

Xin-Jie Song¹, Fei Ye¹, Yao Zhang¹

¹Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology for Agricultural Products, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Liuxia Street Number 318, Hangzhou 310023, PR China.

Food chemistry: X

|November 11, 2024

概括

一种新的光检测方法使用aptamer修饰的纳米颗粒来准确量化猪肉中的 (CLB) 残留物. 这种敏感测试为可靠的食品安全分析提供了0.304 ng/mL的检测极限.

科学领域:

分析化学分析化学
纳米技术纳米技术
生物医学工程生物医学工程

背景情况:

克伦布醇 (CLB) 是由于健康风险而在食品生产中被禁止使用的物质.
对食品中CLB残留物的准确和敏感的检测方法对于公共卫生和监管合规性至关重要.

研究的目的:

开发一种新的光检测方法,用于灵敏和准确地量化克伦布醇 (CLB).
为了利用aptamer修饰的上转化纳米粒子 (UCNPs) 和磁性纳米粒子 (MNPs) 进行CLB检测.

主要方法:

设计了一种三明治试验,使用aptamer功能化的UCNP和MNP来捕获和检测CLB.
使用磁场分离了MNP-CLB-UCNP复合体,随后进行光强度测量.
采用光谱光度测法,对特定激发 (980 nm) 和辐射 (400-800 nm) 的光谱进行了测试.

主要成果:

光强度与CLB度的增加有直接相关性,表明特异性良好.
该方法实现了高灵敏度,CLB的检测极限为0.304 ng/mL.
在猪肉样本中,CLB的恢复率在84%至94.87%之间,证明了准确性.

结论:

开发的光方法提供了一种灵敏,精确和准确的方法,用于量化猪肉等食品矩阵中的CLB残留物.
这种基于aptamer的纳米粒子测定为食品安全监测和禁止物质控制提供了一个有前途的工具.

关键词:

亚普特美尔 (Aptamer) 是一种药物.克伦布醇的使用方法检测方法检测方法磁性纳米颗粒的使用方法升级转换纳米粒子

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