PIMMS-Dash:可访问的分析,查询和可视化高吞吐量转位子插入测序 (TIS) 数据
Adam M Blanchard1, Adam Taylor2, Andrew Warry2
1University of Nottingham, School of Veterinary Medicine and Science, Leicestershire LE12 5RD, United Kingdom.
Computational and structural biotechnology journal
|November 11, 2024
概括
研究人员现在可以很容易地使用PIMMS-Dash,一个新的网络工具来分析转子子插入测序 (TIS) 数据. 这种工具简化了识别健康贡献基因和生成出版质量的数据,而不需要广泛的生物信息学专业知识.
科学领域:
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
- 分子生物学分子生物学
背景情况:
- 高通量转子子体插入测序 (TIS) 数据分析是复杂和耗时的.
- 现有的方法通常需要重要的生物信息专业知识,并产生静态可视化.
- 缺乏可访问的工具阻碍了实时数据查询和对基因功能的理解.
研究的目的:
- 开发一个可访问的基于Web的工具,用于可视化和分析TIS数据.
- 让所有生物信息学技能水平的研究人员能够查询TIS数据集.
- 为了促进在实验系统中对健康有所贡献的基因的识别.
主要方法:
- 创建了一个基于Web的浏览器工具PIMMS-Dash.
- 与TIS数据处理的PIMMS分析管道的集成.
- 实现交互式和可排序表用于数据探索.
主要成果:
- PIMMS-Dash提供了TIS数据的交互可视化.
- 该工具可以轻松识别感兴趣的基因及其对健康的贡献.
- 研究人员可以直接从该工具中生成出版物质量数据.
结论:
- 皮姆斯-达什民主化了TIS数据分析,使其可供更广泛的研究社区使用.
- 该工具通过直观的数据查询来增强对基因健康贡献的理解.
- PIMMS-Dash支持有效生成科学出版物的高质量数据.
更多相关视频
10:34Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR
Published on: February 1, 2013
14.1K
11:36Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
Published on: September 23, 2017
15.9K
相关概念视频
02:57DNA-only Transposons
14.4K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
14.4K
02:13Overview of Transposition and Recombination
15.2K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.2K