相关实验视频

Updated: Jun 7, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

在将CRISPR/Cas技术应用于树木基因组编辑方面的进展和挑战

Solme Pak¹, Chenghao Li¹

¹State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.

Forestry research

|November 11, 2024

概括

集群定期间隔的简短的Palindromic重复 (CRISPR) /CRISPR相关蛋白 (Cas) 技术推进了树基因组编辑以改善特征. 提高CRISPR/Cas的效率对于加速树木繁殖和特征发展至关重要.

科学领域:

植物生物技术植物生物技术
基因组编辑基因组编辑
林业科学林业科学

背景情况:

聚类正规间隔短时间的Palindromic重复 (CRISPR) /CRISPR相关蛋白 (Cas) 系统彻底改变了植物基因组编辑.
克里斯普尔/卡斯工具包能够精确的基因编辑,包括基因和主要编辑,促进作物改进.

研究的目的:

审查CRISPR/Cas在树基因组编辑中的应用现状.
确定阻碍CRISPR/Cas工具包在树木中的有效应用的局限性.
讨论树基因组编辑的未来前景.

主要方法:

审查关于CRISPR/Cas在各种树木物种中的应用现有的文献.
对基因编辑结果的分析,包括indel,base和prime编辑.
对转化方法的评估,重点是以Agrobacterium为媒介的和短暂的转化.

主要成果:

克里斯普尔/卡斯已经成功地应用于众多树种,为生长,新陈代谢和抗压力提供了洞察力.
大多数树研究都专注于初步优化,主要是使用Cas9用于indel突变.
通过Agrobacterium稳定转换是常见的,但过渡转换效率仍然很低,限制了无转基因编辑.

结论:

克里斯普尔/卡斯技术具有极大的潜力,可以加速树木繁殖和特征改进.
为了高效的树基因组编辑,需要进一步研究优化编辑技术,特别是短暂转换.
克服目前的局限性将释放CRISPR/Cas对各种树种的全部潜力.

关键词:

集群定期间隔的短时间的Palindromic重复/CRISPR相关蛋白质.森林树是森林中的树.基因编辑基因编辑基因转换是一种基因转换.核蛋白-蛋白质复合体是一个复合体.

更多相关视频

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

What is Genetic Engineering?

What is Genetic Engineering?

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...