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疾病衍生的循环细胞外囊泡预条件:精确介质干细胞治疗的有希望的策略

Ke Lv¹, Tian Wu¹, Shuyun Liu¹

¹Department of Integrated Traditional Chinese and Western Medicine and NHC Key Laboratory of Transplant Engineering and Immunology, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu 610041, China.

Acta pharmaceutica Sinica. B

|November 11, 2024

概括

用疾病衍生的细胞外囊 (EVs) 预先调配介质干细胞 (MSCs),提高它们的组织修复能力. 这一策略在各种损伤模型中改善了MSC功能和治疗结果.

科学领域:

再生医学是一种再生医学.
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

基于介质干细胞 (MSC) 的疗法显示出再生医学的前景.
提高MSC组织修复功效仍然是一个关键的挑战.
来自疾病状态的循环细胞外囊泡 (EVs) 携带病理信息.

研究的目的:

调查由疾病衍生的循环EV (疾病-EV) 预条件化作为加强MSC组织修复的策略.
探索疾病-EV介导的MSC增强背后的机制.
在各种疾病模型中评估预条件MSCs的治疗疗效.

主要方法:

从肺部或脏组织损伤中收集的血EV.
预先条件培养的MSCs具有疾病-EVs.
分析了MSC基因表达,新陈代谢 (氧化酸化,脂质新陈代谢),增殖,迁移和生长因子的产生.
在急性肺部和损伤模型中评估MSC治疗效果 (抗炎,抗瘤).

主要成果:

疾病-EVs含有不同的分子形状,并诱导MSC中的组织损伤特定基因反应.
疾病-EV预先条件通过代谢重编程加强了MSC的扩散,迁移和生长因子的产生.
在受伤模型中,预先条件的MSC显示出优异的抗炎和抗瘤作用.
在疾病-EV预先条件下没有观察到不良的免疫反应.

结论:

疾病-EV预条件是精确提高MSC组织修复功能的有效策略.
代谢重编程是疾病-EV介导的MSC增强的一个关键机制.
来自疾病的电动汽车作为再生疗法的"现成"产品提供了优势.

关键词:

细胞死亡细胞死亡循环中的细胞外囊泡.炎症炎症是一种炎症.介质细胞干细胞介质细胞干细胞.代谢重编程是一种代谢重编程.预先条件的形成再生医学是一种再生医学.组织损伤组织损伤

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相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...