通过circEEF2/miR-625-5p/TRPM2轴激活刺通路,通过线粒体应激促进前列腺癌细胞的增殖
ChenHui Zhu1, LiJuan Lin2, ChangQing Huang1
1Department of Urology, Central People's Hospital of Zhanjiang, Zhanjiang City, Guangdong Province.
European journal of histochemistry : EJH
|November 11, 2024
概括
循环RNA circEEF2通过激活子通路促进前列腺癌 (PCa). 削弱circEEF2抑制PCa细胞的增殖,迁移和入侵,同时增强细胞亡,揭示了一个新的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 前列腺癌 (PCa) 仍然是一个重要的健康问题,其复杂的潜在分子机制.
- 循环RNAs (circRNAs) 正在成为各种癌症的关键调节者,包括PCa.
- 识别像circEEF2这样的新型分子参与者对于理解PCa病原体至关重要.
研究的目的:
- 阐明circEEF2 (has-circ-0048559) 在前列腺癌发展中的作用.
- 研究circEEF2影响PCa进展的分子机制.
- 探索针对circEEF2/miR-625-5p/TRPM2轴进行治疗干预的潜力.
主要方法:
- 定量实时PCR (RT-qPCR) 来评估circEEF2,miR-625-5p和TRPM2的表达.
- 细胞检测包括CCK-8,殖民地形成,Transwell和流细胞计,以评估增殖,迁移,入侵和亡.
- 双 luciferase 记者和RNA 拉下测试以确认分子相互作用.
- 西方涂抹,JC-1染色,ROS测定和免疫光用于途径和细胞应激分析.
- 在小鼠中的异种移植模型以评估体内瘤生长.
主要成果:
- 在PCa组织和细胞中,CircEEF2表达显著上调.
- 循环EEF2的耗尽抑制了PCa细胞的增殖,迁移和入侵,同时诱导了细胞亡.
- CircEEF2与miR-625-5p直接相互作用,miR-625-5p针对TRPM2.2进行了向.
- 通过miR-625-5p/TRPM2轴激活刺信号通路,CircEEF2促进了PCa的发展,导致线粒体应激增加.
结论:
- 在前列腺癌中,CircEEF2充当了一个关键的瘤性circRNA.
- 环EEF2/miR-625-5p/TRPM2轴在促进PCa进展方面发挥着关键作用.
- CircEEF2诱导的刺路径激活和线粒体应激有助于PCa的发展,这表明circEEF2是潜在的治疗标.
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