在天然发生骨髓瘤的狗中免疫学前转移性
Mikael Kerboeuf1, Kristin Paaske Anfinsen2, Erling Olaf Koppang1
1Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, Norway.
Veterinary and comparative oncology
|November 11, 2024
概括
在患有骨髓瘤 (OS) 的狗中,转移前的位形成显示了转移前肺部骨髓衍生细胞的增加. 这种狗模型为开发针对前转移性的治疗方法提供了洞察力.
科学领域:
- 在瘤学瘤学.
- 兽医医学 兽医医学 兽医医学
- 细胞生物学 细胞生物学
背景情况:
- 转移前利基 (PMN) 形成是癌症转移的一个关键步骤,涉及由瘤衍生因素重塑遥远的器官微环境.
- 狗的骨髓瘤 (OS) 由于共同的生物特征,可以作为人类OS的宝贵翻译模型.
研究的目的:
- 调查自然发生骨髓瘤的狗的肺部前转移性的存在和特征.
- 为了确定骨髓衍生细胞 (BMDCs) 在这种犬类模型中的明显转移之前是否被招募到肺部.
主要方法:
- 多重免疫光染色被用于量化免疫细胞种群,包括巨细胞和单细胞,从OS和健康对照的未经治疗的狗的肺组织中.
- 分析了患有OS的狗的肺组织,分为有肺转移和没有肺转移的狗,并与对照犬进行比较.
主要成果:
- 与对照物种相比,在患有OS的狗的肺部观察到显著增加的CD204+巨细胞,CD206+巨细胞,单细胞和CD11d+骨髓衍生细胞 (BMDCs).
- 在转移前患有OS的狗的肺部观察到总核细胞密度增加.
- 已确立肺转移的狗中CD11d+BMDC数量下降,表明过渡性招募.
结论:
- 这项研究提供了在自然发生的犬骨髓瘤模型中存在前转移性的证据.
- 在患有OS的狗中,骨髓衍生细胞被招募到肺部,先于转移发育.
- 狗骨肉瘤模型适用于评估针对前转移性的新疗法.
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