在接受生育保护的年轻患者中评估卵巢储备的Z-分数
Jasmin Hassan1, Pauliina Damdimopoulou1, Atte Lahtinen2
1Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet; Department of Gynecology and Reproductive Medicine, Karolinska University Hospital.
Journal of visualized experiments : JoVE
|November 11, 2024
概括
在接受生育保护的年轻女性中评估卵巢储备至关重要. 这项研究引入了皮层卵泡密度的Z-score,将患者样本与年龄正常化的标准进行比较,以评估卵巢储备质量.
科学领域:
- 生殖内分泌学 生殖内分泌学
- 在生育能力上.
- 组织病理学 组织病理学
背景情况:
- 女性拥有有限的,不可再生的卵巢卵泡储备.
- 遗传性疾病,医疗治疗和内分泌干扰剂等因素会影响卵巢储备.
- 对于面临性腺毒疗法的人来说,保持生育能力至关重要,而卵巢组织冷保存是前青春期的唯一选择.
研究的目的:
- 提出一种标准化方案,用于评估接受生育保护的年轻女性 (≤25岁) 卵巢组织质量.
- 引入Z-score用于皮层卵泡密度的使用,作为评估卵巢储备的工具.
- 为了将患者的毛囊密度与偏差估计的年龄正常化参考标准进行比较.
主要方法:
- 在冷保存的卵巢皮质组织样本 (~2 mm3) 中,囊泡密度的组织学量化.
- 组织加工:固定,石墨烯嵌入,4μm切割,血素和色素染色,以及幻灯片数字化.
- 使用修改后的公式计算毛囊密度,并根据已建立的年龄规范化参考数据确定Z分数.
主要成果:
- Z-score量化了个体的卵泡密度与年龄正常化平均值的偏差.
- 低于-1.7的Z分数表明卵巢储备减少.
- 该方法为年轻患者的卵巢储备评估提供了定量测量.
结论:
- 通过Z分数评估的皮层卵泡密度是评估年轻女性需要保持生育能力的卵巢储备的有价值指标.
- 该协议有助于为儿科和青少年患者提供生育咨询.
- 标准化组织学量化和Z分数分析可以提高卵巢组织质量的评估.
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