在耐火性突发感神经听力损失中,HIF-1α的上调调节
Wandong She1,2, Ziwen Gao2, Wenyan Zhu2,3
1Department of Otolaryngology-Head and Neck Surgery, The Affiliated Suzhou Hospital of Nanjing University Medical School, Suzhou, China.
概括
低氧诱导因子-1α (HIF-1α) 的激活抑制了基因素脱乙酶2 (HDAC2) 的表达,导致耐火性突然神经感应性听力损失 (SSNHL) 中的葡萄糖皮质体耐药性. HIF-1α可能预测SSNHL治疗结果.
科学领域:
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 耐火性突发感神经听力损失 (SSNHL) 是一个重大的临床挑战.
- 了解SSNHL中葡萄糖皮质体抗性背后的分子机制对于改善治疗策略至关重要.
研究的目的:
- 研究缺氧诱导因子-1α (HIF-1α) 在耐火性SSNHL患者中的作用.
- 探索HIF-1α,基因素脱乙酶2 (HDAC2) 和葡萄皮质激素耐药性之间的关系.
主要方法:
- 三十名耐火性SSNHL患者接受了入门甲基prednisolone输液 (IMP).
- HIF-1α和HDAC2的表达被测量在周围血液单核细胞 (PBMC) 和在氧化应激下体外细胞模型中.
- 在HEI-OC1细胞中进行了基因操纵实验.
主要成果:
- 根据IMP后听力改善,患者被分为葡萄糖皮质激素敏感 (GCS) 和葡萄糖皮质激素耐药 (GCR) 组.
- 在治疗前,HDAC2的水平下降,HIF-1α在所有不耐药的SSNHL患者中增加.
- HIF-1α上调显著降低了HDAC2的表达,特别是在氧化应激下,这表明了葡萄糖皮质醇耐药性的机制.
结论:
- 似乎HIF-1α激活抑制了HDAC2的表达,从而导致耐火性SSNHL的葡萄糖皮质醇耐药性.
- HIF-1α可能作为预测耐火性SSNHL预后的潜在生物标志物.
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