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微生物组产生的SCFA与早产有关,通过 trofhoblast 功能调节

Lulu Meng^1,2, Meng Meng³, Ruonan Zhang²

¹Department of Obstetrics, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

|November 11, 2024

概括

过早分娩 (PTB) 的风险与宫阴道微生物组不平衡以及短链脂肪酸 (SCFA) 增加有关. 这项研究确定了微生物和代谢物标记,以准确预测和诊断PTB.

科学领域:

微生物学微生物学
产科产科产科产科产科
代谢学代谢学代谢学

背景情况:

过早分娩 (PTB) 是婴儿死亡和发病的主要原因,其病因复杂且不完全理解.
宫性阴道微生物组及其代谢物,如短链脂肪酸 (SCFA),与PTB风险有关.
了解这些微生物 - 胎盘相互作用对于开发预测和预防策略至关重要.

研究的目的:

调查宫性阴道微生物组成,SCFA水平和PTB之间的关联.
探索SCFA在胎盘 trofhoblast 细胞中的功能作用.
确定用于预测和诊断PTB的潜在生物标志物.

主要方法:

16S rRNA基因测序和针对性代谢对孕妇的宫和阴道抽样.
在体外使用胎盘 trofhoblast 细胞 (HTR8 / SVneo) 的功能测试.
微生物和代谢数据的综合分析,包括气色谱-质谱.

主要成果:

显著的宫性阴道失调 (乳酸菌减少,无气性细胞增加) 与PTB相关 (P=0.036).
在早产组中发现了较高的酸水平 (P=0.047),与Gardnerella vaginalis.正相关.
一组诊断标记 (怀孕类型,酸,社区状态类型) 准确地预测了PTB.
乙酸盐通过ERK1/2信号传递促进了IL-8,IL-6和热囊细胞迁移/入侵.

结论:

宫性阴道微生物群失生症是PTB的重要病因因素.
宫阴道微生物群和SCFA可用于PTB预测和诊断.
这些发现支持微生物群-胎盘轴理论,并表明微生态干预的潜力.

关键词:

16S rRNA 是一个16S rRNA.在SCFAs中,SCFAs是最重要的.宫和阴道的微生物群怀孕怀孕怀孕怀孕怀孕过早出生过早出生

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