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Updated: Jun 7, 2025

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
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使用光排除测量单分裂酵母细胞体积的高通量测量.

Larisa Venkova1, Daniel García-Ruano1,2, Akanksha Jain1,2

  • 1Institute of Biochemistry and Cellular Genetics, CNRS UMR 5095 and University of Bordeaux, Bordeaux, France.

Methods in molecular biology (Clifton, N.J.)
|November 11, 2024
PubMed
概括

准确测量裂变酵母细胞体积对于理解细胞生物学至关重要. 这项研究引入了一种使用光排除和微流体的新方法,用于精确,高通量细胞体积的Schizosaccharomyces pombe.

关键词:
自动化分析分析自动化分析细胞体积 细胞体积 细胞体积深度学习是一种深度学习.裂变酵母酵母是一种裂变酵母.光异常排除 光异常排除活细胞成像成像技术微流体学 微流体学

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Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
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