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相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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相关实验视频

Updated: Jun 7, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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简单的等离子体编辑方法

Ruben C Petreaca1,2, Hovik J Gasparyan3

  • 1Department of Molecular Genetics, The Ohio State University, Marion, OH, USA.

Methods in molecular biology (Clifton, N.J.)
|November 11, 2024
PubMed
概括
此摘要是机器生成的。

分子克隆技术已经推进了基因功能研究. 一种新的方法SIMPLE (SapI/AarI切口介导等离子体编辑),可以实现精确的等离子体修改,如删除和插入.

关键词:
分子克隆是一种分子克隆.塑编辑 塑编辑局部导向的突变发生.

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CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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Homemade Site Directed Mutagenesis of Whole Plasmids
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CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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科学领域:

  • 分子生物学分子生物学
  • 基因工程是一种基因工程.

背景情况:

  • 分子克隆技术,包括PCR和局部特异性突变发生,对于理解基因功能至关重要.
  • 这些方法在生物研究实验室中被广泛使用.

研究的目的:

  • 介绍和描述一种名为SIMPLE (SapI/AarI切口介导等离子体编辑) 的新型分子克隆技术.
  • 为实施简单克隆方法提供详细说明.

主要方法:

  • 该研究详细介绍了一种新技术,即简单克隆,该技术利用SapI/AarI限制酶进行等离子体编辑.
  • 这种方法促进了高效的删除,短插入 (例如,表位标记) 和精确的突变.

主要成果:

  • 简单的克隆提供了一种精确而有效的方法来修改插曲性等离子体.
  • 该技术适用于各种遗传操作,包括删除,插入和突变.

结论:

  • 简单的克隆代表了分子遗传学家工具箱的宝贵补充.
  • 这种方法提高了生物研究等离子体工程的精度和效率.